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Development of 14 microsatellite markers in the Queensland koala (Phascolarctos cinereus adustus) using next generation sequencing technology.
Conservation Genetics Resources ( IF 0.9 ) Pub Date : 2014-06-01 , DOI: 10.1007/s12686-013-0115-2
Christina T Ruiz-Rodriguez 1 , Yasuko Ishida 1 , Alex D Greenwood 2 , Alfred L Roca 1
Affiliation  

We report the development of 14 new microsatellite markers in the Queensland koala (Phascolarctos cinereus adustus). Ten unrelated Queensland koala individuals from the San Diego Zoo, USA, were genotyped. The number of alleles per locus ranged from 2 to 7, with an average of 5.14 alleles per locus. Across all loci, the average observed and expected heterozygosity values were both 0.69. These polymorphic microsatellite loci will be useful for genetic studies relevant to the conservation of the koala, a species listed as vulnerable.

中文翻译:

使用下一代测序技术在昆士兰考拉 (Phascolarctos cinereus adustus) 中开发 14 个微卫星标记。

我们报告了昆士兰考拉 (Phascolarctos cinereus adustus) 中 14 个新微卫星标记的发展。对来自美国圣地亚哥动物园的 10 只无血缘关系的昆士兰考拉进行了基因分型。每个基因座的等位基因数从 2 到 7 个不等,平均每个基因座有 5.14 个等位基因。在所有基因座中,平均观察到的和预期的杂合度值均为 0.69。这些多态性微卫星位点将有助于与考拉保护相关的遗传研究,考拉是一种被列为易危物种。
更新日期:2019-11-01
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