This article reports on the application of organically modified silica (ORMOSIL) nanoparticles as an efficient in vitro gene delivery system in the recent years. Based on that prime objective, the present study addresses the possible ways to reduce cancers incidence at cellular level. In this context, ORMOSIL nanoparticles had been synthesized and incubated along with pCMV–Myc (3.8 kb) plasmid vector construct carrying p53gene, and transfected into the breast cancer cell line MCF-7 cells. Western blot analysis showed that the p53 protein was significantly expressed in breast cancer cell upon transfection. The confocal and electron microscopic studies further confirmed that the nanoparticles were accumulated in the cytoplasm and the nucleus of the cancer cells transfected with p53 gene. Interesting agarose gel electrophoresis studies revealed that the nanoparticles efficiently complex with pCMV–Myc vector. The anti-cancer properties of p53 were demonstrated by assessing the cell survival and growth rate which showed a positive linear correlation in cancer cells. Whereas, the growth rate was significantly reduced in ORMOSIL/p53/pCMV–Myc transfected breast cancer cells compared to the growth rate of non-transfected cells. The results of this approach using ORMOSIL nanoparticles as a non-viral gene delivery platform have a promising future for use as effective transfection agent for therapeutic manipulation of cancer cells and targeted cancer gene therapy in vivo.

中文翻译：

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开发使用ORMOSIL纳米粒子的体外基因递送系统：分析培养的乳腺癌细胞（MCF-7）中p53基因的表达。

本文报道了近年来有机改性的二氧化硅（ORMOSIL）纳米颗粒作为有效的体外基因递送系统的应用。基于这一主要目标，本研究提出了在细胞水平上降低癌症发生率的可能方法。在这种情况下，已合成ORMOSIL纳米颗粒，并与携带p53基因的pCMV–Myc（3.8 kb）质粒载体构建体一起孵育，并转染到乳腺癌细胞系MCF-7细胞中。Western印迹分析表明，p53蛋白在转染后在乳腺癌细胞中显着表达。共聚焦和电子显微镜研究进一步证实，纳米颗粒聚集在转染了p53基因的癌细胞的细胞质和细胞核中。有趣的琼脂糖凝胶电泳研究表明，纳米颗粒与pCMV–Myc载体有效复合。通过评估细胞存活率和生长率证明了p53的抗癌特性，该率在癌细胞中呈正线性相关。而与未转染细胞相比，ORMOSIL / p53 / pCMV-Myc转染的乳腺癌细胞的生长速度明显降低。使用ORMOSIL纳米颗粒作为非病毒基因递送平台的这种方法的结果，有望用作有效的转染剂，用于体内治疗癌细胞和靶向癌基因治疗。通过评估细胞存活率和生长速率证明了p53的抗癌特性，该细胞在癌细胞中显示出正线性相关性。而与未转染细胞相比，ORMOSIL / p53 / pCMV-Myc转染的乳腺癌细胞的生长速度明显降低。使用ORMOSIL纳米颗粒作为非病毒基因递送平台的这种方法的结果，有望用作有效的转染剂，用于体内癌细胞的治疗操作和靶向癌症基因治疗。通过评估细胞存活率和生长速率证明了p53的抗癌特性，该细胞在癌细胞中显示出正线性相关性。而与未转染细胞相比，ORMOSIL / p53 / pCMV-Myc转染的乳腺癌细胞的生长速度明显降低。使用ORMOSIL纳米颗粒作为非病毒基因递送平台的这种方法的结果，有望用作有效的转染剂，用于体内癌细胞的治疗操作和靶向癌症基因治疗。