The remarkable health benefits of the chemical cocktails occluded within Vites vinefera (grapes) have been broadly used as dietary supplements and as natural pharmaceuticals in the treatment of various diseases including human cancer. Current discovery demonstrates the rapid formation of gold nanoparticles with the phytochemicals present in grapes, which serve a dual role as synergistic reducing agents to reduce gold salts into gold nanoparticles and also as stabilizers to provide a robust coating on the gold nanoparticles in a single step. Furthermore, the grape-generated gold nanoparticles (GAuNPs), have demonstrated remarkable in vitro stability on specific functionalization with peptides (GSH) and thiol-containing compounds (lipoic acid) followed by the induction of cell-specific response. In addition, the grape-generated gold nanoparticles (GAuNPs, GSH-GAuNPs, LA-GAuNPs) have demonstrated remarkable affinity towards human breast cancer cells (HBL-100) in the present study. These studies thus signified the cellular internalization of GAuNPs and its conjugates by transmission electron microscopy through endocytosis into cancer cells. Notably, at higher concentration of gold nanoparticles conjugate, there was an asymmetric accumulation of gold nanoparticles in the periphery of the cell nucleus of the HBL-100 cells which was confirmed by fluorescence microscopy. Other than gold salts, no “manmade” chemicals are used in this truly biogenic, green nanotechnological process which thereby paves the way for outstanding opening for their application in molecular imaging and cancer therapy.

中文翻译：

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从 Vites vinefera 中轻松合成生物相容性金纳米粒子及其针对 HBL-100 细胞的细胞内化。


葡萄中含有的化学混合物具有显着的健康益处，已被广泛用作膳食补充剂和天然药物，用于治疗包括人类癌症在内的各种疾病。目前的发现表明，葡萄中存在的植物化学物质可以快速形成金纳米颗粒，其具有双重作用，既可以作为协同还原剂，将金盐还原成金纳米颗粒，又可以作为稳定剂，一步在金纳米颗粒上提供坚固的涂层。此外，葡萄生成的金纳米粒子（GAuNP）在肽（GSH）和含硫醇化合物（硫辛酸）的特定功能化以及随后诱导细胞特异性反应方面表现出显着的体外稳定性。此外，在本研究中，葡萄生成的金纳米颗粒（GAuNP、GSH-GAuNP、LA-GAuNP）已表现出对人类乳腺癌细胞（HBL-100）的显着亲和力。因此，这些研究通过透射电子显微镜表明 GAuNP 及其缀合物通过胞吞作用进入癌细胞的细胞内化。值得注意的是，在较高浓度的金纳米颗粒缀合物下，HBL-100细胞的细胞核周围存在金纳米颗粒的不对称积累，这一点通过荧光显微镜证实。除了金盐之外，这种真正的生物绿色纳米技术过程中不使用任何“人造”化学物质，从而为其在分子成像和癌症治疗中的应用铺平了道路。