There is need for devices that decrease detection time of food-borne pathogens from days to real-time. In this study, a rapid-detection device is being developed and assessed for potential cytotoxicity. The device is comprised of melt-spun polypropylene coupons coated via oxidative chemical vapor deposition (oCVD) with 3,4-Ethylenedioxythiophene (EDOT), for conductivity and 3-Thiopheneethanol (3TE), allowing antibody attachment. The Ames test and comet assay have been used in this study to examine the toxicity potentials of EDOT, 3TE, and polymerized EDOT-co-3TE. For this study, Salmonella typhimurium strain TA1535 was used to assess the mutagenic potential of EDOT, 3TE and the copolymer. The average mutagenic potential of EDOT, 3TE and copolymer was calculated to be 0.86, 0.56, and 0.92, respectively. For mutagenic potential, on a scale from 0 to 1, close to 1 indicates low potential for toxicity, whereas a value of 0 indicates a high potential for toxicity. The comet assay is a single-cell gel electrophoresis technique that is widely used for this purpose. This assay measures toxicity based on the area or intensity of the comet-like shape that DNA fragments produce when DNA damage has occurred. Three cell lines were assessed; FRhK-4, BHK-21, and Vero cells. After averaging the results of all three strains, the tail intensity of the copolymer was 8.8 % and tail moment was 3.0, and is most similar to the untreated control, with average tail intensity of 5.7 % and tail moment of 1.7. The assays conducted in this study provide evidence that the copolymer is non-toxic to humans.

中文翻译：

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通过Ames试验和彗星试验评估聚合物的毒性潜力，该聚合物用于开发具有现场能力的快速检测设备以分析环境样品。

需要将食源性病原体的检测时间从几天减少到实时的设备。在这项研究中，正在开发一种快速检测装置并评估其潜在的细胞毒性。该设备由熔融纺丝的聚丙烯试样组成，该试样通过氧化化学气相沉积（oCVD）涂覆有3,4-乙二氧基噻吩（EDOT），具有导电性和3-Thiopheneethanol（3TE），可用于抗体附着。Ames试验和彗星试验已用于本研究中，以检查EDOT，3TE和聚合的EDOT-co-3TE的潜在毒性。在本研究中，鼠伤寒沙门氏菌TA1535菌株用于评估EDOT，3TE和共聚物的诱变潜力。EDOT，3TE和共聚物的平均诱变潜力分别为0.86、0.56和0.92。对于致突变性，从0到1，接近1表示毒性低，而值为0表示毒性高。彗星试验是一种广泛用于此目的的单细胞凝胶电泳技术。该测定法是根据DNA损坏发生时DNA片段产生的彗星状形状的面积或强度来测量毒性。评估了三个细胞系；FRhK-4，BHK-21和Vero细胞。将所有三个菌株的结果平均后，共聚物的尾部强度为8.8％，尾部弯矩为3.0​​，与未处理的对照组最相似，平均尾巴强度为5.7％，尾矩为1.7。这项研究中进行的试验提供了证据，证明该共聚物对人体无毒。