A routine computer-assisted sperm analysis is an important diagnostic test in the andrology laboratory. To evaluate the accuracy and precision of the different types of counting chambers for human semen analysis in combination with a computer-assisted semen analyzer (CASA), a quality-control study that compared human sperm analysis results obtained using different counting chambers (Makler chamber, disposable 8-cell GoldCyto chamber, disposable 4-cell Leja chamber, a plain glass slide, and a tissue culture dish cover with a 24 × 24 mm(2) coverslip) in conjunction with the CASA systems was performed. Significantly higher counts of sperm concentration were obtained from the reusable Makler chamber than from the other counting chambers. Sperm motility from drop loaded counting chambers was significantly higher than that of capillary-loaded chambers. A plain glass slide and a tissue culture dish cover used with a coverslip showed rather better performance in semen assessment. Disposable chambers are suitable for routine semen analysis with CASA in a diagnostic andrology setting. With the proper workflow and quality control, a plain glass slide and the tissue culture dish cover are acceptable alternatives for routine counting chambers with CASA as necessary. The type of counting chamber should be specified in test reports.

中文翻译：

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使用计算机辅助精液分析仪比较不同计数室。

常规的计算机辅助精子分析是男科学实验室的重要诊断测试。为了结合计算机辅助精液分析仪（CASA）评估用于人体精液分析的不同类型计数池的准确性和精确度，这项质量控制研究比较了使用不同计数池（Makler室，结合CASA系统执行了一次性8单元GoldCyto室，一次性4单元Leja室，普通玻璃载玻片和带有24×24 mm（2）盖玻片的组织培养皿盖）。从可重复使用的马克勒室获得的精子浓度计数明显高于其他计数室。液滴加载计数室的精子活力显着高于毛细管加载计数室的精子活力。普通玻璃片和用于盖玻片的组织培养皿盖在精液评估中显示出更好的性能。一次性腔室适合在诊断性男科学环境中使用CASA进行常规精液分析。通过适当的工作流程和质量控制，对于需要使用CASA进行常规计数的计数室，可以使用普通载玻片和组织培养皿盖作为替代方案。计数室的类型应在测试报告中规定。对于需要使用CASA进行常规计数的计数室，可以使用普通玻璃载玻片和组织培养皿盖作为替代方案。计数室的类型应在测试报告中规定。对于需要使用CASA进行常规计数的计数室，可以使用普通玻璃载玻片和组织培养皿盖作为替代方案。计数室的类型应在测试报告中指定。