Silver nanoparticles (AgNPs) were biologically synthesized using aqueous extract of Agaricus bisporus fungi. Physicochemical analysis of silver nanoparticles revealed that they are of spherical shape ranged size of 8–20 nm, and their zeta potential equal −7.23 mV. Silver nanoparticles showed a dose-dependent cytotoxic effect on MCF-7 breast cancer cells with LD50 (50 μg/ml). Mice bearing Ehrlich solid tumor treated with AgNPs and exposed to gamma radiation significantly ameliorated superoxide dismutase and catalase activity and reduced glutathione with an increase in malondialdehyde and nitric oxide levels compared to tumor group. Gamma radiation with AgNPs induced apoptotic cell count in Ehrlich solid tumor cells from 68.3 (treated with AgNPs) to 98.1 % (treated with AgNPs with gamma radiation) via a mechanism involved caspase-3. Histological sections of tumor tissue of mice treated with AgNPs showed antiangiogenesis effect of AgNPs. The overall result indicates that AgNPs synergize with gamma radiation, promising a potential combined therapy of cancer.

中文翻译：

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真菌介导的银纳米颗粒的合成和抗肿瘤活性的评估。

使用双孢蘑菇真菌的水性提取物生物合成了银纳米颗粒（AgNPs）。对银纳米颗粒的理化分析表明，它们的球形大小范围为8–20 nm，其zeta电位等于-7.23 mV。银纳米颗粒对LD50（50μg/ ml）的MCF-7乳腺癌细胞显示出剂量依赖性的细胞毒性作用。与肿瘤组相比，用AgNPs处理并暴露于γ射线的荷Ehrlich实体瘤小鼠显着改善了超氧化物歧化酶和过氧化氢酶活性，并降低了谷胱甘肽，同时丙二醛和一氧化氮水平增加。通过涉及caspase-3的机制，用AgNPs进行的γ射线诱导Ehrlich实体肿瘤细胞的凋亡细胞数从68.3（用AgNPs处理）到98.1％（用AgNPs进行γ射线处理）。用AgNPs处理的小鼠的肿瘤组织的组织学切片显示AgNPs的抗血管生成作用。总体结果表明，AgNP与γ射线协同作用，有望成为一种潜在的癌症联合治疗方法。