Normal micelle microemulsion method was utilized for fabrication of organically modified silica (ORMOSIL) nanoparticles. The void and dye-doped nanoparticles were synthesized in nonpolar core of two different surfactants including Aerosol OT and Tween 80. The nanoparticles were characterized using transmission electron microscopy, dynamic light scattering, and zeta potential analysis. Our results revealed that the type of surfactant molecules has a dramatic impact on the size and size distribution range, surface charge, and surface functionalization of the nanoparticles. The particles fabricated using Tween 80 had very smaller size with narrow size distribution and very lower amount of zeta potential. For specific delivery of functionalized nanoparticles to breast cancer cell line SKBR3, overexpressing human epidermal growth factor receptor 2 (HER2), both dye-doped nanoparticles fabricated with Aerosol OT or Tween 80, was conjugated to Herceptin. In vitro studies using fluorescent microscopy demonstrated that the surfactant used for preparation of the nanoparticles can affect the uptake of the particles by cells. The dye-doped functionalized ORMOSIL nanoparticles prepared with Aerosol OT showed better efficiency in the process of active targeting of HER2 receptor. Herceptin-functionalized ORMOSIL nanoparticles can be used for differentiation of HER2-positive from HER2-negative breast cancer cells or specific delivery of therapeutic and diagnostic agents and also other nanoparticles such as magnetic nanoparticles and quantum dots to breast cancer cells.

中文翻译：

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赫赛汀功能化的有机改性二氧化硅纳米粒子对HER2阳性乳腺癌细胞的主动靶向。

普通胶束微乳液法被用于有机改性的二氧化硅（ORMOSIL）纳米粒子的制造。在两种不同的表面活性剂（包括Aerosol OT和Tween 80）的非极性核中合成了孔隙和掺杂染料的纳米粒子。使用透射电子显微镜，动态光散射和zeta电位分析对纳米粒子进行了表征。我们的结果表明，表面活性剂分子的类型对纳米粒子的大小和大小分布范围，表面电荷和表面功能化具有重大影响。使用Tween 80制成的颗粒尺寸非常小，尺寸分布窄，ζ电位非常低。为了将功能化的纳米粒子特异递送至乳腺癌细胞株SKBR3，过度表达的人表皮生长因子受体2（HER2），即用Aerosol OT或Tween 80制成的两种染料掺杂纳米颗粒，都与赫赛汀偶联。使用荧光显微镜的体外研究表明，用于制备纳米颗粒的表面活性剂会影响细胞对颗粒的吸收。用Aerosol OT制备的染料掺杂功能化ORMOSIL纳米颗粒在HER2受体的主动靶向过程中显示出更高的效率。赫赛汀功能化的ORMOSIL纳米颗粒可用于将HER2阳性与HER2阴性乳腺癌细胞区分开来，或将治疗剂和诊断剂以及其他纳米颗粒（如磁性纳米颗粒和量子点）特异递送至乳腺癌细胞。