In this study, we report the preparation, luminescence, and targeting properties of folic acid-CdTe quantum dot conjugates. Water-soluble CdTe quantum dots were synthesized and conjugated with folic acid using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-N-hydroxysuccinimide chemistry. The influence of folic acid on the luminescence properties of CdTe quantum dots was investigated, and no energy transfer between them was observed. To investigate the efficiency of folic acid-CdTe nanoconjugates for tumor targeting, pure CdTe quantum dots and folic acid-coated CdTe quantum dots were incubated with human nasopharyngeal epidermal carcinoma cell line with positive expressing folic acid receptors (KB cells) and lung cancer cells without expression of folic acid receptors (A549 cells). For the cancer cells with positive folate receptors (KB cells), the uptake for CdTe quantum dots is very low, but for folic acid-CdTe nanoconjugates, the uptake is very high. For the lung cancer cells without folate receptors (A549 cells), the uptake for folic acid-CdTe nanoconjugates is also very low. The results indicate that folic acid is an effective targeting molecule for tumor cells with overexpressed folate receptors.

中文翻译：

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叶酸-CdTe 量子点缀合物及其在癌细胞靶向中的应用。


在这项研究中，我们报告了叶酸-CdTe 量子点缀合物的制备、发光和靶向特性。使用 1-乙基-3-(3-二甲基氨基丙基)碳二亚胺-N-羟基琥珀酰亚胺化学合成了水溶性 CdTe 量子点并与叶酸缀合。研究了叶酸对CdTe量子点发光性能的影响，没有观察到它们之间的能量转移。为了研究叶酸-CdTe纳米缀合物的肿瘤靶向效率，将纯CdTe量子点和叶酸包被的CdTe量子点与叶酸受体阳性表达的人鼻咽表皮癌细胞系（KB细胞）和未表达叶酸受体的肺癌细胞一起孵育。叶酸受体（A549 细胞）的表达。对于叶酸受体呈阳性的癌细胞（KB细胞），CdTe量子点的摄取非常低，但对于叶酸-CdTe纳米缀合物的摄取非常高。对于没有叶酸受体的肺癌细胞（A549细胞），叶酸-CdTe纳米缀合物的摄取也非常低。结果表明，叶酸是针对叶酸受体过度表达的肿瘤细胞的有效靶向分子。