In the present work, three Spanish local varieties of Prunus avium (L.), as well as two foreign varieties were studied. The content of total phenols, flavonoids, anthocyanins, glucose and fructose of methanolic extracts from ripe fruits of each variety were analysed. A phytochemical profile of these cultivars was performed by UHPLC-qTOF-MS. The employed chromatographic method allowed a clear and rapid separation of the three main phenolic compound groups present in the extracts: hydroxycinnamic acids, anthocyanins and flavonoids. In addition, the extracts DPPH radical scavenging ability, as well as their capacity to affect xanthine/xanthine oxidase system, were determined. Finally, variations in ROS intracellular concentrations in HepG2 cell line cultures treated with cherry extracts were measured through DCFH-DA assay. All extracts showed a significant inhibitory effect on the xanthine/xanthine oxidase system. Differences between in vitro and in cell culture results evidence the interaction among the phenolic compounds of the extract.

在目前的工作中，研究了三个西班牙本地的樱桃李（L.）和两个外国品种。分析了每个品种成熟果实中甲醇提取物的总酚，类黄酮，花色苷，葡萄糖和果糖的含量。这些品种的植物化学特征通过UHPLC-qTOF-MS进行。所采用的色谱方法可以清晰，快速地分离提取物中存在的三个主要酚类化合物基团：羟基肉桂酸，花色苷和类黄酮。另外，提取物DPPH确定自由基清除能力，以及它们影响黄嘌呤/黄嘌呤氧化酶系统的能力。最后，通过DCFH-DA测定法测量了用樱桃提取物处理的HepG2细胞系培养物中ROS细胞内浓度的变化。所有提取物对黄嘌呤/黄嘌呤氧化酶系统均表现出显着的抑制作用。体外和细胞培养结果之间的差异证明了提取物中酚类化合物之间的相互作用。