A novel rapid single-step cleanup method combined with quick, easy, effective, rugged, and safe (QuEChERS) extraction approach for determining multi-pesticide residues was developed. For the single-step QuEChERS (sin-QuEChERS) cleanup procedure, a specified cartridge was fitted within an extraction centrifuge tube for removing non-target substances. Multi-walled carbon nanotubes (MWCNTs) and Primary Secondary Amine (PSA) mixed with salts functioned as absorbents. Method validation was applied on 47 representative pesticides in pepper, chili peppers and chili sauce by LC-MS/MS and GC–MS/MS detection. Compared to the original QuEChERS method, the Sin-QuEChERS method was fast and convenient without any further vortex or centrifugation. Satisfactory recoveries of most pesticides at two concentration levels were in the range of 70–120% (except for pyrimethanil) with relative standard deviations (RSDs n = 5) lower than 17%. The limit of quantification (LOQs) for the 47 pesticides were 0.01 mg/kg in three matrices. Sin-QuEChERS method was successfully applied to monitor the market multi-residues.

开发了一种新颖的快速单步净化方法，结合了快速，简便，有效，坚固耐用且安全的（QuEChERS）提取方法来确定多种农药残留。对于单步QuEChERS（sin-QuEChERS）清洁程序，将指定的滤芯安装在提取离心管中，以去除非目标物质。与盐混合的多壁碳纳米管（MWCNT）和伯仲胺（PSA）起到吸收剂的作用。通过LC-MS / MS和GC-MS / MS检测方法对胡椒，辣椒和辣椒酱中的47种代表性农药进行了方法验证。与原始QuEChERS方法相比，Sin-QuEChERS方法快速简便，无需进一步涡旋或离心分离。在两个浓度水平下，大多数农药的满意回收率在70–120％（除嘧菌胺外）范围内，相对标准偏差（RSDs = 5）低于17％。在三种基质中，这47种农药的定量限（LOQ）为0.01 mg / kg。Sin-QuEChERS方法已成功应用于监测市场中的多残留物。