We present a ‘hit-and-return’ (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.

中文翻译：

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击发系统可实现高效的时间分辨串行同步加速器晶体学

我们为时间分辨的串行同步加速器晶体学提出了一种“击退”（HARE）方法，其时间分辨率从毫秒到几秒或更长时间。使用固定目标晶体学芯片和转换平台系统中的规则模式，机械地设置定时延迟。捕获与乙酸氟脱卤酶结合的中间结构的光学泵浦探针实验表明，可以短时间（30 ms），中时间（752 ms）和长时间（2,052 ms）收集数据。