Scarless genome editing in human pluripotent stem cells (hPSCs) represents a goal for both precise research applications and clinical translation of hPSC-derived therapies. Here we established a versatile and efficient method that combines CRISPR–Cas9-mediated homologous recombination with positive–negative selection of edited clones to generate scarless genetic changes in hPSCs.

中文翻译：

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人多能干细胞中的高效无痕基因组编辑

人类多能干细胞（hPSC）中的无缝基因组编辑代表了hPSC衍生疗法的精确研究应用和临床翻译的目标。在这里，我们建立了一种通用而有效的方法，将CRISPR–Cas9介导的同源重组与编辑克隆的正负选择相结合，从而在hPSC中产生无痕的遗传变化。