Isoorientin is a C-glycosylated derivative of luteolin and exhibits a number of biological properties. In this study, multiple strategies were adopted to improve isoorientin production from luteolin in Escherichia coli. Isoorientin production was improved substantially by adjusting induction strategies and controlling acetic acid accumulation, with maximum isoorientin production reaching 826 mg/L. Additionally, a novel UDP-glucose synthesis pathway was reconstructed in E. coli through cellobiose phosphorylase-catalyzed phosphorolysis of cellobiose for the production of glucose 1-phosphate, which serves as a precursor in UDP-glucose formation. The results from two mechanisms of UDP-glucose formation in E. coli, cellobiose phosphorolysis and sucrose phosphorolysis, were compared. Increasing the UDP-glucose supply resulted in maximal isoorientin production reaching 1371 mg/L. Finally, isoorientin (1059 mg) was obtained from 1 L of fermentation broth by simple purification steps with a yield of 81.5%. Therefore, this study provides an efficient method for isoorientin production and a novel UDP-glucose synthesis pathway.

中文翻译：

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通过调节诱导策略，控制乙酸并增加大肠杆菌中UDP-葡萄糖的供应，将木犀草素有效地转化为异东方素

异Orientinin是木犀草素的C-糖基化衍生物，具有许多生物学特性。在这项研究中，采取了多种策略来改善大黄素在大肠杆菌中的异Orientin产量。通过调整诱导策略和控制乙酸积累，异Orientin的产量得到了显着提高，最大异Orientin的产量达到826 mg / L。另外，通过纤维二糖磷酸化酶催化的纤维二糖磷酸分解在大肠杆菌中重建了新的UDP-葡萄糖合成途径，以产生葡萄糖1-磷酸，其用作UDP-葡萄糖形成的前体。大肠杆菌中UDP葡萄糖形成的两种机制的结果比较了纤维二糖的磷酸分解和蔗糖的磷酸分解。UDP-葡萄糖供应的增加导致异Orientin的最大产量达到1371 mg / L。最后，通过简单的纯化步骤，从1 L的发酵液中获得了Ororientin（1059 mg），产率为81.5％。因此，本研究提供了一种有效的生产异Orientinin的方法和一条新的UDP葡萄糖合成途径。