A novel fluorescence immunosensor based on Förster resonance energy transfer between nitrogen and sulfur co-doped carbon dot functionalized silica nanospheres and Au@Ag NPs†,New Journal of Chemistry

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A novel fluorescence immunosensor based on Förster resonance energy transfer between nitrogen and sulfur co-doped carbon dot functionalized silica nanospheres and Au@Ag NPs†
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2018-12-08 00:00:00 , DOI: 10.1039/c8nj05349a
Jun-Tao Cao _{1,

2,

3,

4,

5} , Wen-Sheng Zhang _{1,

2,

3,

4,

5} , Hui Wang _{1,

2,

3,

4,

5} , Shu-Hui Ma _{4,

5,

6} , Yan-Ming Liu _{1,

2,

3,

4,

5}

Affiliation

A novel fluorescence (FL) immunosensor for prostate-specific antigen (PSA) detection was developed based on the Förster resonance energy transfer (FRET) between nitrogen and sulfur co-doped carbon dot functionalized silica nanospheres (Si/NS-CDs) and Au@Ag NPs. The strategy utilized the PSA primary antibody labeled luminescent Si/NS-CDs as donors and PSA secondary antibody labeled Au@Ag NPs as acceptors. In the presence of PSA, the bio-affinity of the antigen and antibody caused the Si/NS-CDs and Au@Ag NPs to approach at a close enough distance for the FL intensity of the Si/NS-CDs to decline owing to the FRET effect. By virtue of the excellent optical properties of the Si/NS-CDs nanoprobe, the quantitative analysis of PSA has been successfully realized using both FL spectroscopy and a naked-eye readout. The fluorometric results present the desirable analytical performance for PSA detection in the range from 5.0 pg mL⁻¹ to 10 ng mL⁻¹ with a detection limit of 0.95 pg mL⁻¹ (S/N = 3). Moreover, satisfactory results for the determination of the PSA target in samples of human serum were obtained, showing the great promise of the method for application in biochemical analysis.

中文翻译：

基于氮和硫共掺杂的碳之间福斯特共振能量转移的新型荧光免疫斑点官能化二氧化硅纳米球和Au @银纳米颗粒†

基于氮和硫共掺杂碳点官能化二氧化硅纳米球（Au @）之间的傅斯特共振能量转移（FRET），开发了一种用于前列腺特异性抗原（PSA）检测的新型荧光（FL）免疫传感器。银纳米颗粒。该策略利用标记为发光Si / NS-CD的PSA一抗作为供体，标记为Au @ Ag NPs的PSA二抗作为受体。在存在PSA的情况下，抗原和抗体的生物亲和力导致Si / NS-CD和Au @ Ag NP的接近距离足够近，从而导致Si / NS-CD的FL强度下降。 FRET效果。凭借Si / NS-CDs纳米探针的出色光学性能，使用FL光谱和裸眼读数已成功实现了PSA的定量分析。^-1至10 ng mL^-1，检测极限为0.95 pg mL^-1（S / N = 3）。此外，获得了令人满意的测定人血清样品中PSA靶标的结果，表明该方法在生化分析中的应用前景广阔。

更新日期：2018-12-08

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