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Lysosome-targeted two-photon fluorescent probes for rapid detection of H2S in live cells†
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2018-12-08 00:00:00 , DOI: 10.1039/c8nj05419c Gongchun Li 1, 2, 3, 4, 5 , Saige Ma 5, 6, 7, 8 , Jun Tang 5, 6, 7, 8 , Yong Ye 5, 6, 7, 8
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2018-12-08 00:00:00 , DOI: 10.1039/c8nj05419c Gongchun Li 1, 2, 3, 4, 5 , Saige Ma 5, 6, 7, 8 , Jun Tang 5, 6, 7, 8 , Yong Ye 5, 6, 7, 8
Affiliation
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Two novel two-photon fluorescent probes (BHNP-DA and M2) were designed and synthesized,and show good selectivity and high sensitivity to H2S. The response of the probes to H2S in Tris–HCl buffer was rapid (50 s). The reaction mechanism of the probe BHNP-DA is based on hydrogen sulfide mediated tandem disulfide exchange, followed by a nucleophilic substitution–cyclization reaction and the fluorescence is turned on. The dual-nucleophilicity reaction of H2S could not be observed for the probe M2 as the fluorophore group is linked by an amide and the fluorescence is turned off. Furthermore, the probes were successfully applied for the fluorescence imaging of living MCF-7 cells. The colocalization experiments indicate that the probe BHNP-DA can effectively target lysosomes.
中文翻译：
靶向溶酶体的双光子荧光探针，可快速检测活细胞中的H 2 S †
设计并合成了两种新型的双光子荧光探针（BHNP-DA和M2），它们对H 2 S表现出良好的选择性和高灵敏度。在Tris-HCl缓冲液中，探针对H 2 S的响应迅速（50 s ）。BHNP-DA探针的反应机理基于硫化氢介导的串联二硫化物交换，然后进行亲核取代-环化反应，并开启荧光。探针M2无法观察到H 2 S的双亲核反应因为荧光团通过酰胺连接并且荧光被关闭。此外，该探针已成功地用于活MCF-7细胞的荧光成像。共定位实验表明，探针BHNP-DA可以有效地靶向溶酶体。
更新日期：2018-12-08
中文翻译：
靶向溶酶体的双光子荧光探针，可快速检测活细胞中的H 2 S †
设计并合成了两种新型的双光子荧光探针（BHNP-DA和M2），它们对H 2 S表现出良好的选择性和高灵敏度。在Tris-HCl缓冲液中，探针对H 2 S的响应迅速（50 s ）。BHNP-DA探针的反应机理基于硫化氢介导的串联二硫化物交换，然后进行亲核取代-环化反应，并开启荧光。探针M2无法观察到H 2 S的双亲核反应因为荧光团通过酰胺连接并且荧光被关闭。此外，该探针已成功地用于活MCF-7细胞的荧光成像。共定位实验表明，探针BHNP-DA可以有效地靶向溶酶体。
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