Manufacturers of recombinant proteins frequently face the challenge to satisfy the market needs with existing large scale production capacity. For fed-batch processes, the maximal capacity is given in yield per batch multiplied by the number of runs that can be done per year, thus strongly depending on the duration of the fed-batch run. This short review is focused on starting the fed-batch at high seeding densities, reducing the duration of the fed-batch as an efficient strategy to increase the manufacturing capacity without modifying the production bioreactor. This strategy makes fed-batch processes highly productive from the first day on.

中文翻译：

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大规模细胞培养过程的强化

重组蛋白的制造商经常面临挑战，以现有的大规模生产能力满足市场需求。对于分批补料工艺，最大产能以每批的产量乘以每年可以进行的运行次数给出，因此强烈取决于分批补料运行的持续时间。这篇简短的评论集中在以高播种密度启动补料分批，减少补料分批的持续时间，作为提高生产能力而不改变生产生物反应器的有效策略。从第一天开始，这种策略使分批补料流程的生产效率很高。