Abstract The molecular imprinting ratiometric fluorescence sensor has exhibited great potential in biological and environmental detection because of the combination between the excellent selectivity of molecular imprinting technique and outstanding robustness of ratiometric measurement methods On this basis, two kinds of different well-resolved wavelengths and high-fluorescent carbon dots (CDs) were prepared by various solvent extraction using osmanthus fragrans leaves as carbon source. Then the ratiometric fluorescence sensor (MIPs@rCDs/bCDs@SiO2) has been constructed by the obtained CDs which the red-emission CDs (rCDs) were used to responsive signal and the blue-emission CDs (bCDs) was served as reference signal and applied for highly selective and excellent sensitive detection of tetracycline (TC). Under optimum conditions, the ratiometric fluorescence MIPs@rCDs/bCDs@SiO2 sensor has also exhibited lower detection limit of 1.19 nM and the linear range of 0–50 nM. The ratiometric fluorescence sensor was further successfully employed to the determination of TC in water samples gathered from local river water and tap water, indicating its great worth toward water sample analysis in complicated environments.

中文翻译：

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基于红/蓝碳点的定制分子印迹比例荧光传感器用于超灵敏四环素检测

摘要 分子印迹比率荧光传感器结合分子印迹技术的优异选择性和比率测量方法的出色鲁棒性，在生物和环境检测方面表现出巨大的潜力。在此基础上，两种不同的分辨率良好的波长和高分辨以桂花叶为碳源，采用多种溶剂萃取法制备荧光碳点（CDs）。然后用红光CDs（rCDs）响应信号，蓝光CDs（bCDs）作为参考信号，构建了比例荧光传感器（MIPs@rCDs/bCDs@SiO2）。应用于四环素 (TC) 的高选择性和高灵敏度检测。在最佳条件下，比例荧光 MIPs@rCDs/bCDs@SiO2 传感器的检测下限为 1.19 nM，线性范围为 0-50 nM。该比率荧光传感器进一步成功地用于测定当地河水和自来水中采集的水样中的TC，表明其在复杂环境下的水样分析中具有重要价值。