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GHB614 × T304-40 × GHB119 × COT102 Cotton: Protein Expression Analyses of Field-Grown Samples
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-12-06 00:00:00 , DOI: 10.1021/acs.jafc.8b05395 A-J. Wu 1 , K. Chapman 2 , S. Sathischandra 1 , J. Massengill 1 , R. Araujo 1 , M. Soria 1 , M. Bugas 1 , Z. Bishop 1 , C. Haas 1 , B. Holliday 3 , K. Cisneros 1 , J. Lor 1 , C. Canez 1 , S. New 1 , S. Mackie 1 , D. Ghoshal 1 , L. Privalle 1 , P. Hunst 1 , K. Pallett 4
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-12-06 00:00:00 , DOI: 10.1021/acs.jafc.8b05395 A-J. Wu 1 , K. Chapman 2 , S. Sathischandra 1 , J. Massengill 1 , R. Araujo 1 , M. Soria 1 , M. Bugas 1 , Z. Bishop 1 , C. Haas 1 , B. Holliday 3 , K. Cisneros 1 , J. Lor 1 , C. Canez 1 , S. New 1 , S. Mackie 1 , D. Ghoshal 1 , L. Privalle 1 , P. Hunst 1 , K. Pallett 4
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Food and feed safety assessment is not enhanced by performing protein expression analysis on stacked trait products. The expression levels of six proteins in cotton matrices from four single cotton events and three conventionally stacked trait cotton products are reported. Three proteins were for insect control; two proteins confer herbicide tolerance; and one protein was a transformation-selectable marker. The cotton matrices were produced at three U.S., five Brazil, and two Argentina field trials. Similar protein expression was observed for all six proteins in the stacked trait products and the single events. However, when two copies of the bar gene were present in the stacked trait products, the expression level of phosphinothricin acetyl transferase herbicide tolerance was additive. Conventional breeding of genetically engineered traits does not alter the level or pattern of expression of the newly introduced proteins, except when multiple copies of the same transgene are present.
中文翻译：
GHB614×T304-40×GHB119×COT102棉花：田间种植样品的蛋白质表达分析
通过对堆叠性状产品进行蛋白质表达分析，无法增强食品和饲料安全性评估。报道了来自四个单次棉花事件和三种常规堆叠性状棉花产品的棉花基质中六种蛋白质的表达水平。三种蛋白质可用于昆虫控制。两种蛋白质赋予除草剂耐受性；一种蛋白质是转化选择标记。棉基质是在三个美国，五个巴西和两个阿根廷田间试验中生产的。对于堆叠性状产物和单个事件中的所有六个蛋白质，观察到相似的蛋白质表达。但是，当两个副本的时候吧在堆叠的性状产物中存在基因，膦丝菌素乙酰转移酶除草剂耐受性的表达水平是累加的。基因工程性状的常规育种不会改变新引入蛋白质的表达水平或表达模式，除非存在相同转基因的多个拷贝。
更新日期：2018-12-06
中文翻译：
GHB614×T304-40×GHB119×COT102棉花：田间种植样品的蛋白质表达分析
通过对堆叠性状产品进行蛋白质表达分析，无法增强食品和饲料安全性评估。报道了来自四个单次棉花事件和三种常规堆叠性状棉花产品的棉花基质中六种蛋白质的表达水平。三种蛋白质可用于昆虫控制。两种蛋白质赋予除草剂耐受性；一种蛋白质是转化选择标记。棉基质是在三个美国，五个巴西和两个阿根廷田间试验中生产的。对于堆叠性状产物和单个事件中的所有六个蛋白质，观察到相似的蛋白质表达。但是，当两个副本的时候吧在堆叠的性状产物中存在基因，膦丝菌素乙酰转移酶除草剂耐受性的表达水平是累加的。基因工程性状的常规育种不会改变新引入蛋白质的表达水平或表达模式，除非存在相同转基因的多个拷贝。
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