A nanoflow liquid chromatography high resolution mass spectrometry method for the quantification of mycotoxins in nuts has been developed. Two strategies based on QuEChERS methodology were evaluated. Thus, EMR-lipid was compared with a conventional mixture of PSA and C18 dispersive solid phase extraction sorbents which have been commonly used in this type of matrices as sample clean-up. The results showed that the use of EMR-lipid reduced more effectively matrix components, achieving a negligible matrix effect for all mycotoxins studied in peanut, pistachio and almond. The proposed method was validated in line with SANTE guidelines using EMR-Lipid as dispersive solid phase extraction sorbent. The lowest concentration level were between 0.05 and 5 μg kg⁻¹, being lower than the maximum levels established by the current legislation. Recovery rates ranged from 75% to 98% was obtained in all sample studied, achieving also satisfactory precision with RSD values lower than 19% in all cases.

已经开发了一种用于定量坚果中霉菌毒素的纳流液相色谱高分辨率质谱法。对基于QuEChERS方法的两种策略进行了评估。因此，将EMR-脂质与PSA和C18分散固相萃取吸附剂的常规混合物进行了比较，后者在这类基质中通常用作样品净化。结果表明，使用EMR-脂质可更有效地减少基质成分，对于花生，开心果和杏仁中研究的所有霉菌毒素，基质效应均可以忽略不计。使用EMR-Lipid作为分散固相萃取吸附剂，根据SANTE指南对提出的方法进行了验证。最低浓度水平在0.05至5μgkg ^-1之间，低于当前法律规定的最高水平。在所研究的所有样品中，回收率均在75％至98％之间，并且在所有情况下RSD值均低于19％时，也获得了令人满意的精度。