Postoperative adhesion is a relevant clinical problem that causes a variety of clinical complications after abdominal surgery. The objective of this study is to develop a liquid-type antiadhesion agent and evaluate its efficacy in preventing tissue adhesion in a rat peritoneal adhesion model. The liquid-type agent was prepared by submicron-sized emulsification of C17 glycerin ester (C17GE), squalene, pluronic F127, ethanol, and water with a high-pressure homogenizer. The primary component was C17GE, which is an amphiphilic lipid of one isoprenoid-type hydrophobic chain and can form two phases of self-assembly nonlamellar liquid crystals. The C17GE agent consisted of nanoparticles with an internal inverted hexagonal phase when evaluated by small-angle X-ray scattering (SAXS) and cryo-transmission electron microscopy (cryo-TEM). Upon contact with the biological tissue, this agent formed a thin membrane with a bioadhesive property. After this agent was applied to a sidewall injury of rats, it showed a percentage average of adhesion significantly less than that obtained with the Seprafilm® antiadhesion membrane in a rat model. Additionally, the retention of the agent prolonged at the applied site in the peritoneal cavity of rats. In conclusion, the C17GE agent is promising as an antiadhesion material. STATEMENT OF SIGNIFICANCE: Postoperative adhesion remains a common adverse effect. Although various materials have been investigated, there are few products commercially available to prevent adhesion. For the sheet-type agent, it is inconvenient to be applied through small laparotomy, especially in laparoscopic surgery. Additionally, the liquid-type agent currently used requires a complicated procedure to spray at the targeted site. Our liquid-type antiadhesion agent can form liquid crystals and act as a thin membrane-like physical barrier between the peritoneum and tissues to prevent adhesion. Indeed, the antiadhesion agent used in our present study significantly prevents adhesion compared with the antiadhesion membrane most used clinically. Moreover, our agent is highly stable by itself and easy to use in laparoscopic surgery, thus leading to a promising new candidate as an antiadhesion material.

中文翻译：

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类异戊二烯型脂质的C17甘油酯形成非层状液晶的抗粘附作用。

术后粘连是一个相关的临床问题，在腹部手术后会引起多种临床并发症。这项研究的目的是开发一种液体型抗粘连剂，并评估其在大鼠腹膜粘连模型中预防组织粘连的功效。通过使用高压均化器对C17甘油酯（C17GE），角鲨烯，普朗尼克F127，乙醇和水进行亚微米级乳化来制备液体型试剂。主要成分是C17GE，它是一条类异戊二烯型疏水链的两亲脂质，可形成两相自组装非层状液晶。当通过小角X射线散射（SAXS）和低温透射电子显微镜（cryo-TEM）评估时，C17GE试剂由具有内部倒置六方相的纳米颗粒组成。与生物组织接触后，该试剂形成具有生物粘附特性的薄膜。将这种药物应用于大鼠的侧壁损伤后，其粘附百分比平均值明显低于在大鼠模型中使用Seprafilm®抗粘附膜获得的粘附百分比平均值。另外，该试剂的保留在大鼠腹膜腔中的施用部位处延长。总之，C17GE试剂有望用作抗粘连材料。重要性声明：术后粘连仍然是常见的不良反应。尽管已经研究了各种材料，但是市场上很少有可防止粘附的产品。对于片状剂，通过小腹剖腹术特别是在腹腔镜手术中应用是不方便的。此外，当前使用的液体型试剂需要复杂的过程才能在目标部位喷涂。我们的液体型抗粘连剂可以形成液晶，并充当腹膜和组织之间的薄膜状物理屏障，以防止粘连。确实，与临床上最常用的抗粘膜相比，我们本研究中使用的抗粘剂显着阻止了粘连。此外，我们的药剂本身具有很高的稳定性，并且易于在腹腔镜手术中使用，因此成为有前途的抗粘连材料新候选人。与临床上最常用的抗粘膜相比，我们本研究中使用的抗粘剂显着阻止了粘连。此外，我们的药剂本身具有很高的稳定性，并且易于在腹腔镜手术中使用，因此成为有前途的新候选物作为抗粘连材料。与临床上最常用的抗粘膜相比，我们本研究中使用的抗粘剂显着阻止了粘连。此外，我们的药剂本身具有很高的稳定性，并且易于在腹腔镜手术中使用，因此成为有前途的新候选物作为抗粘连材料。