Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive,Journal of Agricultural and Food Chemistry

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Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-12-05 00:00:00 , DOI: 10.1021/acs.jafc.8b05093
Young-Chul Joo ₁ , Young Jin Ko ₁ , Seung Kyou You ₁ , Sang Kyu Shin ₁ , Jeong Eun Hyeon ₂ , Almisned Shuaa Musaad ₁ , Sung Ok Han ₁

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Taurine is a biologically and physiologically valuable food additive. However, commercial taurine production mainly relies on environmentally harmful chemical synthesis. Herein, for the first time in bacteria, we attempted to produce taurine in metabolically engineered Corynebacterium glutamicum. The taurine-producing strain was developed by introducing cs, cdo1, and csad genes. Interestingly, while the control strain could not produce taurine, the engineered strains successfully produced taurine via the newly introduced metabolic pathway. Furthermore, we investigated the effect of a deletion strain of the transcriptional repressor McbR gene on taurine production. As a result, sulfur accumulation and l-cysteine biosynthesis were reinforced by the McbR deletion strain, which further increased the taurine production by 2.3-fold. Taurine production of the final engineered strain Tau11 was higher than in other previously reported strains. This study demonstrated a potential approach for eco-friendly biosynthesis as an alternative to the chemical synthesis of a food additive.

中文翻译：

在谷氨酸棒杆菌中创建新途径来生产牛磺酸作为食品添加剂

牛磺酸是具有生物学和生理价值的食品添加剂。然而，商业牛磺酸的生产主要依赖于对环境有害的化学合成。本文中，我们首次尝试在代谢工程化的谷氨酸棒杆菌中产生牛磺酸。牛磺酸生产菌株是通过引入cs，cdo1和csad基因开发的。有趣的是，尽管对照菌株不能产生牛磺酸，但工程菌株通过新引入的代谢途径成功产生了牛磺酸。此外，我们研究了转录阻遏物McbR基因缺失菌株对牛磺酸产生的影响。其结果是，硫的积累和升-半胱氨酸的生物合成通过McbR缺失菌株得到加强，这进一步将牛磺酸的产量提高了2.3倍。最终工程菌株Tau11的牛磺酸产量高于其他先前报告的菌株。这项研究证明了一种潜在的生态友好型生物合成方法，可以替代食品添加剂的化学合成方法。

更新日期：2018-12-05

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