Identification of Almond (Prunus dulcis) Vicilin As a Food Allergen,Journal of Agricultural and Food Chemistry

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Identification of Almond (Prunus dulcis) Vicilin As a Food Allergen
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-12-04 00:00:00 , DOI: 10.1021/acs.jafc.8b05290
Huilian Che _{1,

2} , Yuzhu Zhang ₂ , Shu-Chen Lyu ₃ , Kari C. Nadeau ₃ , Tara McHugh ₂

Affiliation

Almond is one of the tree nuts listed by U.S. FDA as a food allergen source. A food allergen identified with patient sera has been debated to be the 2S albumin or the 7S vicilin. However, neither of these proteins has been defined as a food allergen. The purpose of this study was to clone, express, and purify almond vicilin and test whether it is a food allergen. Western blot experiment was performed with 18 individual sera from patients with double-blind, placebo-controlled clinical almond allergy. The results showed that 44% of the sera contained IgE antibodies that recognized the recombinant almond vicilin, indicating that it is an almond allergen. Identifying this and additional almond allergens will facilitate the understanding of the allergenicity of seed proteins in tree nuts and their cross-reactivity.

中文翻译：

杏仁（Prunus dulcis）Vicilin作为食物过敏原的鉴定。

杏仁是美国FDA列为食物过敏原的坚果之一。鉴定为患者血清的食物过敏原被争论为2S白蛋白或7S vicilin。但是，这些蛋白质都没有被定义为食物过敏原。这项研究的目的是克隆，表达和纯化杏仁豌豆球蛋白，并测试它是否是食物过敏原。用双盲，安慰剂控制的临床杏仁变态反应患者的18个个体血清进行了蛋白质印迹实验。结果显示44％的血清包含识别重组杏仁豌豆球蛋白的IgE抗体，表明它是杏仁过敏原。识别这种和其他杏仁过敏原将有助于理解坚果中种子蛋白的过敏原及其交叉反应性。

更新日期：2018-12-04

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