Synthesis and Biological Uses of A3B Type Water‐Soluble Phthalocyanine Alternate to Alcian Blue,ChemistrySelect

当前位置： X-MOL 学术 › ChemistrySelect › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Synthesis and Biological Uses of A3B Type Water‐Soluble Phthalocyanine Alternate to Alcian Blue
ChemistrySelect ( IF 1.9 ) Pub Date : 2018-12-04 , DOI: 10.1002/slct.201803371
Mukaddes Özçeşmeci ₁ , Serap Sancar‐Baş ₂ , Barbaros Akkurt ₁ , Esin Hamuryudan ₁ , Şehnaz Bolkent ₂

Affiliation

This study describes the synthesis and characterization of new non‐symmetric cationic phthalocyanine derivatives and their capability for use in staining of microscopic normal and pathological tissue sections, polyacrylamide gel staining, and spectrophotometric analyses. These new synthesized compounds have specifically stained the glycosaminoglycans (GAGs) in the matrix of cartilage tissue of the trachea. In addition, the mucus produced by the stomach, the small intestine and the colon tissue were stained with non‐symmetrical cationic phthalocyanines comparable to the Alcian Blue 8GX. In addition, the determination of GAGs and glycoproteins in polyacrylamide gels has also been evaluated. Besides, the usage of these compounds to spectrophotometrically measure GAGs in biological fluids was also investigated. We have found that 6 and 7 are good alternatives to Alcian Blue 8GX due to comparable staining performance and interaction with biological molecules, especially acidic components such as acidic GAGs and mucin. These dyes have good solubility with quaternary ammonium groups effecting their binding performance to biological molecules.

中文翻译：

A3B型水溶性酞菁替代阿尔辛蓝的合成及生物学用途

这项研究描述了新的非对称阳离子酞菁衍生物的合成和表征，以及它们在显微镜下正常和病理组织切片的染色，聚丙烯酰胺凝胶染色和分光光度分析中的应用能力。这些新合成的化合物已对气管软骨组织基质中的糖胺聚糖（GAGs）进行了特殊染色。此外，用与阿尔辛蓝8GX相当的非对称阳离子酞菁将胃，小肠和结肠组织产生的粘液染色。此外，还评估了聚丙烯酰胺凝胶中GAG和糖蛋白的含量。此外，还研究了这些化合物在分光光度法中测量生物液体中GAG的用途。我们发现6和7是Alcian Blue 8GX的良好替代品，因为它们具有可比的染色性能以及与生物分子（尤其是酸性组分，例如酸性GAG和粘蛋白）的相互作用。这些染料与季铵基团具有良好的溶解性，从而影响它们与生物分子的结合性能。

更新日期：2018-12-04

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11