This paper discusses one of the major concerns in pesticide residue analysis: the matrix effect related to gas chromatography (GC), which can adversely affect quantification. In this study, a comparison of approaches for dealing with the matrix effect was investigated for 236 pesticides in complex matrices, including dried herbs (Centaurea cyanus L., Matricaria chamomilla L., Thymus vulgaris L.) and dried fruit (currants, chokeberry), using a modified QuEChERS method and GC–MS/MS analysis. Three approaches were evaluated: (i) using matrix-matched calibration, (ii) adding a mixture of analyte protectants (APs) to every extract or (iii) injection prior to GC–MS/MS analysis. Finally, minimization of the matrix effect to the acceptable levels of −20 to 20% for over 80% of investigated pesticides was found when APs mixture was injected at the beginning of the sequence. In this approach, the matrix effects were significantly weaker for some pesticides than when matrix-matched calibration was used.

本文讨论了农药残留分析中的主要问题之一：与气相色谱（GC）有关的基质效应，这可能会对定量产生不利影响。在这项研究中，对处理复杂基质中的236种农药的基质效应的方法进行了比较，其中包括干草（Centaurea cyanus L. ，Matricaria chamomilla L. ，百里香（Thymus vulgaris）L.）和干果（黑醋栗，苦莓），使用改良的QuEChERS方法和GC-MS / MS分析。评价了三种方法：（i）使用基质匹配的校准，（ii）在每种提取物中添加分析物保护剂（APs）的混合物，或（iii）在进行GC-MS / MS分析之前进样。最后，当在序列开始时注入AP混合物时，发现对于超过80％的被调查农药，基质效应最小化至可接受的-20至20％的水平。用这种方法，与使用基质匹配的校准方法相比，某些农药对基质的影响要弱得多。