A Spectroscopic Study of the Interaction between Cyanine Dyes with Different Skeleton Structures and Transferrin,ChemistrySelect

当前位置： X-MOL 学术 › ChemistrySelect › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

A Spectroscopic Study of the Interaction between Cyanine Dyes with Different Skeleton Structures and Transferrin
ChemistrySelect ( IF 1.9 ) Pub Date : 2018-12-04 , DOI: 10.1002/slct.201802649
Yunhe Lang ₁ , Lei Shi ₁ , Ling Lan ₂ , Zheng Zhao ₁ , Qianfan Yang ₃ , Lei Chen ₁ , Xiaoran Sun ₁ , Yalin Tang ₂ , Xiufeng Zhang ₁

Affiliation

Human serum transferrin (hTf) has been exploited as a bio‐carrier for targeted drugs to cancer cells where transferrin receptors are expressed at high levels. In this study, cyanine dyes DMSA and DMSB with a similar main core structure were selected to evaluate the effects of heterocycle on binding with hTf by spectroscopic methods. Fluorescence spectral results have shown that DMSB, which contains a selenazole ring, had a strong affinity binding with transferrin, up to 10⁴‐fold higher than DMSA, which contains a thiazole ring. This difference may be attributed to the larger molecular volume of selenazole compared with DMSA. Binding distance between cyanine dyes and hTf demonstrated that the non‐radioactive energy transfer mechanism was also involved in the fluorescence quenching of protein. Furthermore, DMSB‐binding gave rise to a greater decrease of the α‐helix content of hTf than DMSA suggesting that hTf, which shows a looser structure binding with DMSB, increased polarity around the tryptophan residues of hTf, which was confirmed by circular dichroism and synchronous fluorescence spectroscopy. The study provides a certain theoretical basis for the design of cyanine dyes as biomolecular probe to target drugs for hTf.

中文翻译：

骨架结构不同的花青染料与转铁蛋白相互作用的光谱研究

人血清转铁蛋白（hTf）已被用作靶向药物的癌细胞的生物载体，癌细胞以高水平表达转铁蛋白受体。在这项研究中，选择花青染料DMSA和DMSB具有相似的主要核心结构，以通过光谱方法评估杂环对与hTf结合的影响。荧光光谱结果表明，含有亚硒唑环的DMSB与转铁蛋白具有很强的亲和力结合，最高可达10 ⁴－是含有噻唑环的DMSA的-倍。与DMSA相比，这种差异可能归因于硒唑的分子体积更大。花青染料与hTf之间的结合距离表明，非放射性能量转移机制也与蛋白质的荧光猝灭有关。此外，与DMSA相比，DMSB结合导致hTf的α-螺旋含量降低更大，这表明hTf（与DMSB结合的结构较松散）在hTf色氨酸残基周围增加了极性，这已通过圆二色性和同步荧光光谱。该研究为花青染料作为hTf靶向药物的生物分子探针的设计提供了一定的理论基础。

更新日期：2018-12-04

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11