Abstract Selective extraction of hydrophilic compounds is a challenging task for analytical laboratories. In an attempt to address this issue, application of titanium dioxide nanoparticles as a dispersive solid phase extraction sorbent was investigated. In the study, three model analytes were used for method optimization: flavin mononucleotide, thiamine monophosphate and thiamine pyrophosphate. The influence of various parameters on sorption and desorption process was evaluated. Under optimized conditions, the sorption was performed in a presence of 1 M formic acid (pH 1.9) for 10 min under vortex stirring providing efficiency up to 94%. The influence of salt content in sample matrix was found to be negligible while the organic solvent presence slightly improved the sorption efficiency. Elution of analytes from TiO2 sorbent was obtained with both aqueous ammonia and phosphate buffer (pH 11.8) solutions. Under optimized conditions dispersive solid phase extraction method was shown to provide high selectivity with extraction recoveries >89.0% for studied compounds. The susceptibility of the method on various matrices was assayed for extraction of model analytes from enriched tap water, milk, apple juice and human urine samples. Moreover, the methodology was applied for phospholipids removal from pharmaceutical formulation (lecithin microemulsion) of propofol. Due to the utilization of titanium dioxide nanoparticles, the cost of sample preparation step was estimated to be about 0.1 Euro per sample, providing >100-fold reduction of the price for typical analysis using titanium dioxide microspheres.

中文翻译：

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使用二氧化钛纳米粒子对亲水性化合物进行低成本和绿色分散固相萃取

摘要 亲水性化合物的选择性萃取是分析实验室的一项具有挑战性的任务。为了解决这个问题，研究了二氧化钛纳米颗粒作为分散固相萃取吸附剂的应用。在该研究中，三种模型分析物用于方法优化：黄素单核苷酸、硫胺一磷酸和硫胺焦磷酸。评估了各种参数对吸附和解吸过程的影响。在优化条件下，在 1 M 甲酸 (pH 1.9) 的存在下，在涡旋搅拌下进行吸附 10 分钟，提供高达 94% 的效率。发现样品基质中盐含量的影响可以忽略不计，而有机溶剂的存在略微提高了吸附效率。用氨水和磷酸盐缓冲液 (pH 11.8) 溶液从 TiO2 吸附剂中洗脱分析物。在优化条件下，分散固相萃取方法显示出高选择性，研究化合物的萃取回收率 >89.0%。分析了该方法对各种基质的敏感性，以从富集的自来水、牛奶、苹果汁和人尿样品中提取模型分析物。此外，该方法还用于从丙泊酚的药物制剂（卵磷​​脂微乳液）中去除磷脂。由于使用了二氧化钛纳米颗粒，样品制备步骤的成本估计约为每个样品 0.1 欧元，使用二氧化钛微球进行典型分析的价格降低了 100 倍以上。