Abstract In this paper, three kinds of natural polymer materials, silk fibroin (SF), gelatin (GT), and nano-hydroxyapatite (n-HA), are mixed as 3D printing bioink to mimic protein polysaccharide and collagen fibers in natural articular cartilage. By changing the SF content, SF/GT/n-HA composite scaffolds with different ratios are prepared using 3D bioprinting technology. The microstructure and morphology, biological properties and mechanical properties of composite scaffolds are characterized. The results show that the printing precision of the bioink with 10% SF is best, and the composite scaffold with 10% SF also exhibits better mechanical properties, whose tensile elastic modulus is 10.60 ± 0.32 MPa and the compression elastic modulus is 1.22 ± 0.06 MPa. These studies are helpful to understand the interaction between SF, GT and n-HA, and provide a theoretical basis for the preparation of better silk fibroin-based composite scaffolds.

中文翻译：

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3D生物打印SF/GT/n-HA复合支架技术及性能研究

摘要 本文将丝素蛋白 (SF)、明胶 (GT) 和纳米羟基磷灰石 (n-HA) 三种天然高分子材料混合作为 3D 打印生物墨水，以模拟天然关节软骨中的蛋白质多糖和胶原纤维。 . 通过改变SF含量，利用3D生物打印技术制备了不同比例的SF/GT/n-HA复合支架。表征了复合支架的微观结构和形貌、生物学特性和力学性能。结果表明，含10%SF的bioink打印精度最好，含10%SF的复合支架也表现出较好的力学性能，其拉伸弹性模量为10.60±0.32MPa，压缩弹性模量为1.22±0.06MPa . 这些研究有助于理解 SF、GT 和 n-HA 之间的相互作用，