Milk protein, in particular native whey protein, is of interest to dairy manufacturers as a measure of functional and nutritional quality. However, quantification of soluble whey protein (SP) is time consuming; giving rise to the need to develop rapid, accurate, and portable at-line process analytical technology. In this study, the performance of a fluorescence-based analyser(_F) (Amaltheys II, Spectralys Innovations, France) was evaluated for quantification of SP_F and whey protein nitrogen index (WPNI)_F in skim milk, whey protein concentrate and infant formula powders. Rehydration of powders prior to analysis was a key factor for ensuring repeatability and reproducibility. A comparison of the analyser with reference methods for SP_F and WPNI_F resulted in coefficient of determination (R²) > 0.993 for both SP_Kjeldahl method and WPNI_GEA. The results show the fluorescence-based analyser to be rapid, compact, and accurate device, suited for providing reliable support to dairy ingredient and infant formula manufacturers.

Industrial relevance

The fluorescence based analysis investigated in this article is suitable for application in the dairy industry where it can be used as a rapid, at-line PAT tool for both liquid and powder samples. The technology has the potential to replace well-established methods for measurement of soluble protein. The main benefit to industry is the ability to respond more rapidly to variations in soluble protein without compromising on the accuracy associated with more time consuming methods.

中文翻译：

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基于荧光的分析仪，可快速测定乳制品成分和婴儿奶粉中的可溶性蛋白质含量

乳蛋白，特别是天然乳清蛋白，是乳制品生产商所关注的功能和营养质量指标。但是，可溶性乳清蛋白（SP）的定量分析非常耗时。因此需要开发快速，准确和便携式的在线过程分析技术。在这项研究中，评估了基于荧光的分析仪（_F）（Amaltheys II，Spectralys Innovations，法国）的性能，以定量分析脱脂牛奶，乳清蛋白浓缩物和婴儿配方奶中的SP _F和乳清蛋白氮指数（WPNI）_F。粉末。分析前粉末的再水化是确保可重复性和重现性的关键因素。分析仪与SP _F参考方法的比较和WPNI _˚F导致决定系数（R ²）> 0.993两个SP_凯氏定氮法及WPNI _GEA。结果表明，基于荧光的分析仪是一种快速，紧凑和准确的设备，适合为乳制品成分和婴儿配方奶粉制造商提供可靠的支持。

行业相关性

本文研究的基于荧光的分析适用于乳制品行业，在该行业中，它可用作液体和粉末样品的快速在线PAT工具。该技术有可能取代公认的可溶性蛋白质测量方法。工业上的主要好处是能够对可溶性蛋白质的变化做出更快的响应，而不会影响更多耗时的方法的准确性。