The effect of vacuum packing and ambient storage conditions on the stability of the β-carotene in the transgenic Golden Rice® lines was studied. The β-carotene was quantified using RP-HPLC at bimonthly intervals for a period of six months. The β-carotene concentration in the genotypes analyzed ranged from 7.13 to 22.81 µg/g of endosperm. The transgene being the same in all the genotypes, variation in the β-carotene concentration reflects on the genetic background of the rice variety and the transgene position that governed the differential accumulation of β-carotene. It was observed that in the absence of light, oxidative degradation is higher followed by thermal degradation. Weibull model with higher R² best explained the degradation kinetics of β-carotene in Golden Rice® lines across all the storage conditions. The knowledge generated through this study can be utilized in devising an effective delivery system for Golden Rice® to the consumer.

研究了真空包装和环境储存条件对转基因GoldenRice®品系中β-胡萝卜素稳定性的影响。使用RP-HPLC每隔两个月对β-胡萝卜素进行定量，为期六个月。所分析基因型中的β-胡萝卜素浓度范围为7.13至22.81 µg / g胚乳。转基因在所有基因型上都是相同的，β-胡萝卜素浓度的变化反映了水稻品种的遗传背景和决定β-胡萝卜素差异积累的转基因位置。观察到，在没有光的情况下，氧化降解较高，随后发生热降解。具有较高R ^2的Weibull模型最好的解释是在所有存储条件下，GoldenRice®品系中β-胡萝卜素的降解动力学。通过这项研究获得的知识可用于为客户设计有效的GoldenRice®递送系统。