Monitoring Metallo‐Macromolecular Assembly Equilibria by Ion Mobility‐Mass Spectrometry,Macromolecular Rapid Communications

当前位置： X-MOL 学术 › Macromol. Rapid Commun. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Monitoring Metallo‐Macromolecular Assembly Equilibria by Ion Mobility‐Mass Spectrometry
Macromolecular Rapid Communications ( IF 4.2 ) Pub Date : 2018-11-30 , DOI: 10.1002/marc.201800667
Kevin J. Endres ₁ , Ting‐Zheng Xie ₁ , Sourav Chakraborty ₁ , Chad Hoopingarner ₁ , Chrys Wesdemiotis ₂

Affiliation

Ion mobility–mass spectrometry (IM‐MS) allows the separation of isomeric and isobaric species on the basis of their size, shape, and charge. The fast separation timescale (ms) and high sensitivity of these measurements make IM‐MS an ideally suitable method for monitoring changes in macromolecular structure, such as those occurring in interconverting terpyridine‐based metallosupramolecular self‐assemblies. IM‐MS is used to verify the elemental composition (size) and architecture (shape) of the self‐assembled products. Additionally, this article demonstrates its applicability to the elucidation of concentration‐driven association–dissociation (fusion–fission) equilibria between isobaric structures. IM‐MS enables both quantitative separation and identification of the interconverting complexes as well as derivation of the corresponding equilibrium constants (i.e., thermodynamic information) from extracted IM‐MS abundance data.

中文翻译：

通过离子淌度质谱法监测金属-大分子组装平衡

离子淌度质谱（IM-MS）可以根据其大小，形状和电荷对同分异构和同量异位的物种进行分离。这些测量的快速分离时标（ms）和高灵敏度使IM‐MS成为监测大分子结构变化的理想方法，例如在互转换基于三联吡啶的金属超分子自组装过程中发生的变化。IM‐MS用于验证自组装产品的元素组成（尺寸）和结构（形状）。此外，本文证明了其在阐明等压结构之间浓度驱动的缔合-解离（融合-裂变）平衡方面的适用性。

更新日期：2018-11-30

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11