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Extraction and characterization of dextran from Leuconostoc pseudomesenteroides YB-2 isolated from mango juice
Carbohydrate Polymers ( IF 10.7 ) Pub Date : 2018-11-29 , DOI: 10.1016/j.carbpol.2018.11.092
Guangbin Ye , Genliang Li , Changli Wang , Bo Ling , Ruirui Yang , Suoyi Huang

An exopolysaccharide (EPS)-producing strain of YB-2 isolated from mango juice was identified as Leuconostoc pseudomesenteroides. The molecular weight (Mw) of this EPS was 7.67×105 Da. Gas chromatography (GC) analysis confirmed the presence of only glucose monomers. Fourier transform infrared (FT-IR) spectroscopy and nuclear magnetic resonance (NMR) spectra displayed the glucan nature of the EPS with 96.8% α-(1→6) and 3.2% branching α-(1→3) linkages. Scanning electron microscopy (SEM) showed smooth surfaces and compact structure. The water solubility index (WSI) and water-holding capacity (WHC) of dextran were 97.48±2.46% and 287.51±7.93%, respectively. The rheological analysis of dextran elucidated a non-Newtonian pseudoplastic behavior. The dextran revealed an inhibitory activity against Escherichia coli and Staphylococcus aureus with minimal inhibitory concentrations (MIC) of 2.0 mg/mL and 3.0 mg/mL, respectively.



中文翻译:

从芒果汁中分离到青叶豆YB-2中右旋糖酐的提取与表征

从芒果汁中分离到的一种产生外多糖(EPS)的YB-2菌株被鉴定为假肠球菌。该EPS的分子量(Mw)为7.67×10 5。大。气相色谱(GC)分析证实仅存在葡萄糖单体。傅里叶变换红外(FT-IR)光谱和核磁共振(NMR)光谱显示出EPS的葡聚糖性质,具有96.8%的α-(1→6)和3.2%的分支α-(1→3)键。扫描电子显微镜(SEM)显示光滑的表面和紧凑的结构。葡聚糖的水溶性指数(WSI)和持水量(WHC)分别为97.48±2.46%和287.51±7.93%。葡聚糖的流变学分析阐明了一种非牛顿的假塑性行为。葡聚糖显示出对大肠杆菌金黄色葡萄球菌的抑制活性,最小抑制浓度分别为2.0 mg / mL和3.0 mg / mL。

更新日期:2018-11-30
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