Bioactive peptides can provide health benefits due to different mechanisms. The aims of the present study are to produce bioactive peptides from bovine and goat milk subjected to the proteolytic activity of Aspergillus oryzae and Aspergillus flavipes enzymes, as well as to assess their putative antimicrobial and antioxidant activity. Bioactive peptides were successfully generated from proteases of fungi cultivated in solid-state fermentation. The generated peptides were effective against all tested bacteria and fungi. There was antioxidant activity, up to 92.5% DPPH reduction and ORAC stabilization at 52.5 μmol μL⁻¹ of Trolox Equivalent. The generation of milk-specific sequences peptides in the samples was obtained through 2D-PAGE fractioning followed by mass spectrometry (ESI-MS/MS). Based on results in the present study, milk bioactive peptides presenting broad antimicrobial action and antioxidant activity spectra can be cost-effectively produced through solid-state fermentation. The herein addressed approach can be valuable for the pharmaceutical and food industries.

由于不同的机制，生物活性肽可以提供健康益处。本研究的目的是从牛乳和山羊乳中生产具有生物活性的肽，该肽具有米曲霉和黄曲霉酶的蛋白水解活性，并评估其推定的抗微生物和抗氧化活性。从在固态发酵中培养的真菌的蛋白酶成功地产生了生物活性肽。产生的肽对所有测试的细菌和真菌均有效。在52.5μmolμL ^-1下具有抗氧化活性，DPPH降低高达92.5％，ORAC稳定Trolox等效值。通过2D-PAGE分馏，然后进行质谱分析（ESI-MS / MS），获得了样品中牛奶特异性序列肽的生成。根据本研究的结果，可以通过固态发酵经济高效地生产出具有广泛抗菌作用和抗氧化活性谱的牛奶生物活性肽。本文解决的方法对于制药和食品工业而言可能是有价值的。