Nanoscale Topography-Rigidity Correlation at the Surface of T Cells.,ACS Nano

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Nanoscale Topography-Rigidity Correlation at the Surface of T Cells.
ACS Nano ( IF 15.8 ) Pub Date : 2018-12-03 , DOI: 10.1021/acsnano.8b06366
Yair Razvag _{1,

2} , Yair Neve-Oz ₂ , Eilon Sherman ₂ , Meital Reches ₁

Affiliation

The mechanical properties of cells affect their function, in sensing, development, and motility. However, the rigidity of the cell surface and its correlation to its local topography remain poorly understood. Here, we applied quantitative imaging AFM to capture high-resolution force maps at the surface of nonadherent T cells. Using this method, we found a positive topography-rigidity correlation at the cells' surface, as opposed to a negative correlation at the surface of adherent cells. We then used 3D single-molecule localization microscopy of the membrane and cortical actin and an actin-perturbing drug to implicate actin involvement in the positive rigidity-topography correlation in T cells. Our results clearly reveal the variability of cell-surface rigidity and its underlying mechanism, showing a functional role for cortical actin in the PM protrusions of T cells, since they are locally more rigid than their surroundings. These findings suggest the possible functional role of membrane protrusions as mechanosensors.

中文翻译：

T细胞表面的纳米级地形刚度相关性。

细胞的机械性质会影响其在感觉，发育和运动中的功能。但是，细胞表面的刚性及其与局部形貌的相关性仍然知之甚少。在这里，我们应用定量成像原子力显微镜以捕获非粘附性T细胞表面的高分辨率力图。使用这种方法，我们发现细胞表面的形貌-刚度呈正相关，而贴壁细胞表面的呈负相关。然后，我们使用了膜和皮质肌动蛋白以及一种肌动蛋白扰动药物的3D单分子定位显微镜，以暗示肌动蛋白参与T细胞的正刚性-形貌相关性。我们的结果清楚地揭示了细胞表面刚度的变化及其潜在机理，由于它们在局部上比周围环境坚硬，因此在T细胞的PM突起中显示了皮质肌动蛋白的功能。这些发现表明膜突起作为机械传感器的可能功能作用。

更新日期：2018-11-28

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