In this work, chondroitin sulfate (CS) was extracted from chicken leg bone soup using the heat-resin static adsorption extraction (HSAE) method. The HSAE method was optimized as follows: resin dosage, 10%; adsorption time, 4.3 h; eluent concentration, 2 M; eluent time, 1.3 h, under which the yield of CS1 from the bone soup reached 0.14% and the recovery rate was 67.35%. CS2, as reference, was obtained from the ends of chicken leg bone using enzymatic method. CS1 and CS2, together with other glycosaminoglycans, were confirmed using agarose-gel electrophoresis. The average molecular weight of CS1 and CS2 was 35.81 kDa and 37.18 kDa, respectively. The structures of CS1 and CS2 were compared using Fourier-transform infrared spectroscopy and high-performance liquid chromatography, and no significant difference was observed. Overall, the HSAE method was proposed to be a promising approach for the coproduction of CS and bone soup.

在这项工作中，采用热树脂静态吸附提取（HSAE）方法从鸡腿骨汤中提取了硫酸软骨素（CS）。HSAE方法的优化如下：树脂用量为10％；树脂用量为10％。吸附时间4.3小时；洗脱液浓度：2 M；洗脱时间为1.3 h，骨汤中CS1的收率达到0.14％，回收率达到67.35％。作为参考，使用酶法从鸡腿骨的末端获得CS2。使用琼脂糖凝胶电泳确认了CS1和CS2以及其他糖胺聚糖。CS1和CS2的平均分子量分别为35.81 kDa和37.18 kDa。使用傅里叶变换红外光谱和高效液相色谱比较了CS1和CS2的结构，但未观察到显着差异。全面的，