We herein report a practical one-step glucuronidation method by biotransformation using Streptomyces sp. SANK 60895. This novel direct method of biotransformation has been shown to be more practical and scalable for glucuronidation than previously reported chemical and enzymatic procedures given its simplicity, high β-selectivity, cost-effectiveness, and reproducibility. We applied the present method to the synthesis of acyl glucuronide and hydroxy-β-glucuronide of mycophenolic acid and compound 4, respectively. This method was also shown to be applicable to the N-glucuronidation of various compounds.

中文翻译：

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通过生物转化进行实用的一步法葡萄糖醛酸化

我们在这里报告了一种实用的一步法葡糖醛酸化方法，该方法通过使用链霉菌属的生物转化进行。SANK60895。由于其简单，高β-选择性，成本效益和可重复性，与以前报道的化学和酶促方法相比，这种新颖的直接生物转化方法已被证明对葡萄糖醛酸苷化更为实用和可扩展。我们将本方法分别应用于麦考酚酸和化合物4的酰基葡糖醛酸苷和羟基-β-葡糖醛酸苷的合成。还表明该方法适用于各种化合物的N-葡萄糖醛酸化。