Hyaluronic acid (HA) is widely investigated due to its high potential for wound dressing applications. The fabrication of biomimetic HA-based scaffolds by electrospinning is thus extensively studied. However, HA is often dissolved in toxic organic solvents to allow the efficient production of electrospun nanofibers. Indeed, although HA is soluble in water, its ionic nature leading to long-range electrostatic interactions and the presence of counter ions induce a dramatic increase of the viscosity of aqueous HA solutions without insuring enough chain entanglements necessary for a stable and efficient electrospinning. In this study, biocompatible insoluble HA-based nanofibers were fabricated by electrospinning in pure water. To this end, poly(vinyl alcohol) (PVA) was added as a carrier polymer and it was found that the addition of hydroxypropyl-β-cyclodextrin (HPβCD) stabilized the process of electrospinning and led to the efficient formation of uniform nanofibrous scaffolds. An in situ crosslinking process of the scaffolds is also proposed, insuring a whole fabrication process without any toxicity. Furthermore, the beneficial presence of HPβCD in the HA-based scaffolds paves the way for wound dressing applications with controlled drug encapsulation-release properties. As a proof of concept, naproxen (NAP), a non-steroidal anti-inflammatory drug was chosen as a model drug. NAP was impregnated into the scaffolds either in aqueous solution or under supercritical CO₂. The resulting functional scaffolds showed a regular drug release profile along several days without losing the fibrous structure. This study proposes a simple approach to form stable HA-based nanofibrous scaffolds embedding HPβCD using water as the only solvent, enabling the development of safe functional wound dressings.

透明质酸（HA）由于其在伤口敷料应用中的巨大潜力而​​被广泛研究。因此，广泛研究了通过电纺丝制造仿生HA基支架。但是，HA通常溶解在有毒的有机溶剂中，以有效生产静电纺丝纳米纤维。的确，尽管HA溶于水，但其离子性质导致长距离静电相互作用和抗衡离子的存在，导致HA水溶液的粘度急剧增加，而没有确保稳定有效的静电纺丝所需的足够的链缠结。在这项研究中，通过在纯水中静电纺丝制备了生物相容性不溶基于HA的纳米纤维。为此，加入聚乙烯醇（PVA）作为载体聚合物，发现添加羟丙基-β-环糊精（HPβCD）可以稳定静电纺丝过程，并有效地形成均匀的纳米纤维支架。还提出了支架的原位交联过程，以确保整个制造过程没有任何毒性。此外，HPβCD在基于HA的支架中的有益存在为具有受控的药物包封释放特性的伤口敷料应用铺平了道路。作为概念的证明，选择非甾体类抗炎药萘普生（NAP）作为模型药物。在水溶液中或在超临界CO下将NAP浸渍到支架中 此外，HPβCD在基于HA的支架中的有益存在为具有受控的药物包封释放特性的伤口敷料应用铺平了道路。作为概念的证明，选择非甾体类抗炎药萘普生（NAP）作为模型药物。在水溶液中或在超临界CO下将NAP浸渍到支架中 此外，HPβCD在基于HA的支架中的有益存在为具有受控的药物包封释放特性的伤口敷料应用铺平了道路。作为概念的证明，选择非甾体类抗炎药萘普生（NAP）作为模型药物。在水溶液中或在超临界CO下将NAP浸渍到支架中₂。所得的功能性支架在几天内显示出规则的药物释放曲线，而不会失去纤维结构。这项研究提出了一种简单的方法来形成稳定的基于HA的纳米纤维支架，该支架使用水作为唯一的溶剂嵌入HPβCD，从而能够开发安全的功能性伤口敷料。