当前位置: X-MOL 学术Microchem. J. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A chemiluminescence aptasensor based on Cu/Co nanorods for microcystin-RR detection
Microchemical Journal ( IF 4.9 ) Pub Date : 2019-03-01 , DOI: 10.1016/j.microc.2018.11.031
Bingru Liu , Xiaohua Li , Sumin Liu , Xu Hun

Abstract In this work, a chemiluminescence (CL) aptasensor was designed for detection of microcystin-RR (MC-RR) by introducing Cu/Co nanorods acting as CL catalyzer. Cu/Co nanorods were firstly synthesized throughout a hydrothermal method. Then the thiolated MC-RR aptamer was immobilized on the surface of Cu/Co nanorods, which was employed for recognizing of MC-RR target. In the presence of MC-RR, the conformational change of MC-RR aptamer was happened via recognizing between MC-RR and its aptamer. The luminol CL was catalyzed by Cu/Co nanorods that influenced by conformational change of MC-RR aptamer. The formed MC-RR/aptamer composite prevented the diffusion channel of luminol substrate toward the Cu/Co nanorods surface and restrained the Cu/Co nanorods catalyzing luminol CL. And the intensity of CL depended on the concentration of MC-RR. Under optimal condition, the CL aptasensor had a linear range in 0.1 to 70 nM and a low detection limit of 3.3 × 10−11 M for MC-RR. This study provided a significant method for MC-RR detection in real samples with excellent selectivity.

中文翻译:

基于Cu/Co纳米棒的化学发光适体传感器用于微囊藻毒素-RR检测

摘要 在这项工作中,通过引入充当 CL 催化剂的 Cu/Co 纳米棒,设计了一种化学发光 (CL) 适体传感器,用于检测微囊藻毒素-RR (MC-RR)。Cu/Co纳米棒首先通过水热法合成。然后将巯基化的 MC-RR 适配体固定在 Cu/Co 纳米棒的表面,用于识别 MC-RR 目标。在MC-RR存在的情况下,MC-RR适体的构象变化是通过MC-RR与其适体之间的识别而发生的。鲁米诺CL由受MC-RR适体构象变化影响的Cu/Co纳米棒催化。形成的MC-RR/适配体复合物阻止了鲁米诺底物向Cu/Co纳米棒表面的扩散通道,并抑制了Cu/Co纳米棒对鲁米诺CL的催化作用。并且CL的强度取决于MC-RR的浓度。在最佳条件下,CL 适体传感器的线性范围为 0.1 至 70 nM,MC-RR 的检测下限为 3.3 × 10−11 M。该研究为实际样品中的 MC-RR 检测提供了一种具有优异选择性的重要方法。
更新日期:2019-03-01
down
wechat
bug