Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed, resolution, and depth. Since its introduction decades ago, the technique has grown to offer myriad implementations, each with its own strengths and weaknesses. We discuss these, aiming to provide a practical guide for biologists and to highlight which approach is best suited to a given application.

中文翻译：

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更快，更清晰，更深入：用于生物成像的结构化照明显微镜

结构照明显微镜（SIM）可以对活体标本进行快速，超分辨率（SR）成像。我们回顾了SR-SIM的最新技术进展，重点是成像速度，分辨率和深度。自从几十年前引入以来，该技术已经发展为提供了无数种实现，每种实现都有其自身的优点和缺点。我们讨论这些内容，旨在为生物学家提供实用的指南，并强调哪种方法最适合给定的应用。