Mechanochemical-Assisted Extraction of Active Alkaloids from Plant with Solid Acids,ACS Sustainable Chemistry & Engineering

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Mechanochemical-Assisted Extraction of Active Alkaloids from Plant with Solid Acids
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2018-11-26 00:00:00 , DOI: 10.1021/acssuschemeng.8b02902
Shuling Wang ₁ , Rui Zhang ₁ , Xiaoyu Song ₁ , Mengmeng Wei ₁ , Tian Xie ₁ , Jun Cao _{1,

2}

Affiliation

In this work, a novel mechanochemical-assisted extraction (MCAE) coupled with the ultra-high performance liquid chromatography (UHPLC) method was developed to extract two alkaloids with solid acids from Stephania tetrandra S. Moore. To further understand the influence of extraction parameters on the MCAE process, the response surface methodology was applied for systematic investigation. The results showed that the highest yield of alkaloids was obtained within 15 min of the total extraction time under the optimal conditions: oxalic acid (20 wt %), grinding plant materials for 5 min, and the liquid/solid ratio of 20 mL/g. Mechanochemical treatment resulted in the destruction of cell walls and increased the total contact surface area due to the smooth surface changing to an open porous structure. Accordingly, the developed extraction method showed advantages of being more ecofriendly, faster, and more efficient than conventional extraction methods. Meanwhile, the extractant was analyzed by UHPLC. The obtained LODs for fangchinoline and tetrandrine were 0.013 and 0.012 μg/mL and good linearity with correlation coefficient of determination higher than 0.9991. Moreover, spiked recoveries were in the range of 101.03%–103.81%. This proves that mechanochemistry processing provides a sensitive, highly effective, and environmentally friendly method.

中文翻译：

固体酸的机械化学辅助提取植物中活性生物碱

在这项工作中，开发了一种新颖的机械化学辅助萃取（MCAE）和超高效液相色谱（UHPLC）方法，以从Stephania tetrandra S. Moore中提取具有固体酸的两种生物碱。。为了进一步了解提取参数对MCAE过程的影响，将响应面方法应用于系统研究。结果表明，在最佳条件下，在总提取时间的15分钟内可获得最高的生物碱产量：草酸（20 wt％），研磨植物材料5 min，液/固比为20 mL / g 。机械化学处理导致细胞壁的破坏并由于光滑的表面变为开放的多孔结构而增加了总的接触表面积。因此，所开发的提取方法显示出比常规提取方法更环保，更快和更有效的优点。同时，通过UHPLC分析萃取剂。所获得的防己诺林碱和粉防己碱的检测限为0.013和0。012μg/ mL且线性良好，相关系数高于0.9991。此外，加标回收率在101.03％–103.81％的范围内。这证明了机械化学加工提供了一种灵敏，高效且环境友好的方法。

更新日期：2018-11-26

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