Integration of marker-free selection of single cells at a wireless electrode array with parallel fluidic isolation and electrical lysis†,Chemical Science

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Integration of marker-free selection of single cells at a wireless electrode array with parallel fluidic isolation and electrical lysis†
Chemical Science ( IF 7.6 ) Pub Date : 2018-11-26 00:00:00 , DOI: 10.1039/c8sc04804e
Min Li _{1,

2,

3,

4} , Robbyn K. Anand _{1,

2,

3,

4}

Affiliation

We present integration of selective single-cell capture at an array of wireless electrodes (bipolar electrodes, BPEs) with transfer into chambers, reagent exchange, fluidic isolation and rapid electrical lysis in a single platform, thus minimizing sample loss and manual intervention steps. The whole process is achieved simply by exchanging the solution in a single inlet reservoir and by adjusting the applied voltage at a pair of driving electrodes, thus making this approach particularly well-suited for a broad range of research and clinical applications. Further, the use of BPEs allows the array to be scalable to increase throughput. Specific innovations reported here include the incorporation of a leak channel to balance competing flow paths, the use of ‘split BPEs’ to create a distinct recapture and electrical lysis point within the reaction chamber, and the dual purposing of an ionic liquid as an immiscible phase to seal the chambers and as a conductive medium to permit electrical lysis at the split BPEs.

中文翻译：

无线电极阵列上无标记选择的单细胞集成，具有并行流体隔离和电裂解功能†

我们目前在一个无线电极（双极电极，BPE）阵列上集成了选择性单细胞捕获功能，并可以在一个平台上转移到腔室中，进行试剂交换，流体隔离和快速电裂解，从而最大程度地减少了样品损失和手动干预步骤。只需在单个入口容器中交换溶液并通过调节一对驱动电极上的施加电压即可简单地完成整个过程，从而使该方法特别适合广泛的研究和临床应用。此外，BPE的使用允许阵列可扩展以增加吞吐量。这里报道的具体创新包括合并泄漏通道以平衡竞争的流路，

更新日期：2018-11-26

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