The potential use of cellulose nanofibers (CNFs) as a reinforcing agent in banana starch-based nanocomposite films was investigated. CNFs were isolated from banana peel (Musa paradisiaca) by enzymatic hydrolysis. Banana starch-based nanocomposite films were prepared with CNFs using the casting method. CNFs effect on cell viability and on nanocomposite films properties’ was investigated. The cytotoxicity of CNFs was assessed on Caco-2 cell line. CNFs were not cytotoxic at 50-2000 μg/mL. However, CNFs above 2000 μg/mL significantly decreased cell viability. Topography analysis showed that the incorporation of CNFs modified the film structure. The nanocomposites exhibited a complex structure due to strong interactions between CNFs and starch matrix, promoting a remarkable improvement on mechanical and water barrier properties, opacity and UV light barrier compared to the control film. CNFs can offer a great potential as reinforcing material for starch-based nanocomposite films, producing a value-added food packaging from a waste material.

研究了纤维素纳米纤维（CNFs）在香蕉淀粉基纳米复合薄膜中作为增强剂的潜在用途。从香蕉皮中分离出CNFs（Musa paradisiaca）通过酶促水解。香蕉淀粉基纳米复合薄膜是通过流延法用CNF制备的。研究了CNF对细胞活力和纳米复合膜性能的影响。在Caco-2细胞系上评估CNF的细胞毒性。CNF在50-2000μg/ mL下无细胞毒性。但是，高于2000μg/ mL的CNF会显着降低细胞活力。形貌分析表明，CNF的引入改变了膜的结构。纳米复合材料由于CNF和淀粉基体之间的强相互作用而显示出复杂的结构，与控制膜相比，促进了机械和水阻隔性能，不透明性和紫外线阻隔性的显着改善。CNF作为淀粉基纳米复合薄膜的增强材料可以提供巨大的潜力，