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Efficient Xylitol Production from Cornstalk Hydrolysate Using Engineered Escherichia coli Whole Cells
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-11-22 00:00:00 , DOI: 10.1021/acs.jafc.8b04666 Ziyue Chang 1 , Dong Liu 1 , Zhengjiao Yang 1 , Jinglan Wu 1 , Wei Zhuang 1 , Huanqing Niu 1 , Hanjie Ying 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-11-22 00:00:00 , DOI: 10.1021/acs.jafc.8b04666 Ziyue Chang 1 , Dong Liu 1 , Zhengjiao Yang 1 , Jinglan Wu 1 , Wei Zhuang 1 , Huanqing Niu 1 , Hanjie Ying 1
Affiliation
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Economic transformation of lignocellulose hydrolysate into valued-added products is of particular importance for energy and environmental issues. In this study, xylose reductase and glucose dehydrogenase were cloned into plasmid pETDuet-1 and then simultaneously expressed in Escherichia coli BL21(DE3), which was used as whole-cell catalyst for the first time to convert xylose into xylitol coupled with gluconate production. When tested with reconstituted xylose and glucose solution, 0.1 g/mL cells could convert 1 M xylose and 1 M glucose completely and produced 145.81 g/L xylitol with a yield of 0.97 (g/g) and 184.85 g/L gluconic acid with a yield of 1.03 (g/g) in 24 h. Subsequently, the engineered cells were applied in real cornstalk hydrolysate, which generated 30.88 g/L xylitol and 50.89 g/L gluconic acid. The cells were used without penetration treatment, and CaCO3 was used to effectively regulate the pH during the production, which further saved costs.
中文翻译：
使用工程化大肠杆菌全细胞从玉米秸秆水解物中高效生产木糖醇
将木质纤维素水解产物经济转化为高附加值产品对于能源和环境问题尤为重要。在这项研究中，木糖还原酶和葡萄糖脱氢酶被克隆到质粒pETDuet-1中，然后在大肠杆菌中同时表达BL21（DE3）首次用作全细胞催化剂，可将木糖转化为木糖醇，并产生葡萄糖酸盐。当用重构的木糖和葡萄糖溶液测试时，0.1 g / mL细胞可以完全转化1 M木糖和1 M葡萄糖，并产生145.81 g / L木糖醇，产量为0.97（g / g）和184.85 g / L葡萄糖酸，其中24小时内产率为1.03（g / g）。随后，将工程细胞应用于真正的玉米秸秆水解物中，生成的水解木糖醇含量为30.88 g / L，葡萄糖酸含量为50.89 g / L。这些电池未经渗透处理即可使用，而CaCO 3可在生产过程中有效调节pH值，从而进一步节省了成本。
更新日期：2018-11-22
中文翻译：
使用工程化大肠杆菌全细胞从玉米秸秆水解物中高效生产木糖醇
将木质纤维素水解产物经济转化为高附加值产品对于能源和环境问题尤为重要。在这项研究中，木糖还原酶和葡萄糖脱氢酶被克隆到质粒pETDuet-1中，然后在大肠杆菌中同时表达BL21（DE3）首次用作全细胞催化剂，可将木糖转化为木糖醇，并产生葡萄糖酸盐。当用重构的木糖和葡萄糖溶液测试时，0.1 g / mL细胞可以完全转化1 M木糖和1 M葡萄糖，并产生145.81 g / L木糖醇，产量为0.97（g / g）和184.85 g / L葡萄糖酸，其中24小时内产率为1.03（g / g）。随后，将工程细胞应用于真正的玉米秸秆水解物中，生成的水解木糖醇含量为30.88 g / L，葡萄糖酸含量为50.89 g / L。这些电池未经渗透处理即可使用，而CaCO 3可在生产过程中有效调节pH值，从而进一步节省了成本。
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