Abstract An efficient, facile and green synthesis of zinc oxide nanoparticles (ZnO NPs) using Tecoma castanifolia leaf extract was reported. ZnO NPs was characterized by UV–Vis spectroscopy, TEM, EDX, XRD and FTIR. Phytochemical constituents of T. castanifolia leaf extract were analyzed by GC–MS. UV–Vis absorption showed SPR band at 370–400 nm which confirms the formation of ZnO NPs. TEM analysis exhibits spherical shape with size 70–75 nm and XRD results revealed the hexagonal phase of wurtzite structure. FTIR spectra confirmed the presence of O H, C H, amide-I, II groups, C O bond and metal‑oxygen groups. The presence of bioactive phytochemical constituents in the methanolic extracts of T. castanifolia was identified by GC–MS. An excellent antibacterial activity was observed for both Gram positive and Gram negative bacteria. Results of antioxidant activity showed that increase in concentration of ZnO nanoparticles increases the radical scavenging activity. Anticancer activity with IC50 value as 65 μg/mL which conferred better cytotoxic effects of ZnO NPs on proliferation of A549 cell line. The present study revealed that the pharmacologically active compounds present in the green synthesized ZnO nanoparticles pave the way to lead its effective application in biomedical and nano-drug delivery systems.

中文翻译：

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使用栗叶提取物绿色合成氧化锌纳米颗粒：表征和评价其抗氧化、杀菌和抗癌活性

摘要 报道了使用栗叶提取物高效、简便和绿色合成氧化锌纳米颗粒 (ZnO NPs) 的方法。ZnO NPs 通过紫外-可见光谱、TEM、EDX、XRD 和 FTIR 进行表征。通过 GC-MS 分析 T. castanifolia 叶提取物的植物化学成分。UV-Vis 吸收在 370-400 nm 处显示 SPR 带，这证实了 ZnO NPs 的形成。TEM 分析显示大小为 70-75 nm 的球形，XRD 结果显示纤锌矿结构的六方相。FTIR 光谱证实存在 OH、CH、酰胺-I、II 基团、CO 键和金属氧基团。通过 GC-MS 鉴定了栗叶甲醇提取物中生物活性植物化学成分的存在。对革兰氏阳性菌和革兰氏阴性菌均观察到极好的抗菌活性。抗氧化活性的结果表明，ZnO 纳米颗粒浓度的增加会增加自由基清除活性。抗癌活性的 IC50 值为 65 μg/mL，这赋予了 ZnO NPs 对 A549 细胞系增殖更好的细胞毒作用。本研究表明，绿色合成的 ZnO 纳米粒子中存在的药理活性化合物为其在生物医学和纳米药物递送系统中的有效应用铺平了道路。