In this study, a polysaccharide, BP, was produced by Bacillus subtilis using burdock oligofructose as a carbon source. The polysaccharide BP was isolated from burdock fermented through B. subtilis by hot water extraction, ethanol precipitation, anion exchange and gel filtration chromatography. The molecular weight of BP was determined to be 1.533 × 10⁷ g/mol, and is composed of mannose, rhamnose, glucose, galactose, and arabinose in the ratio of 1:0.12:17.16:0.69:1.10. Furthermore, BP is made up of a backbone structure of (1→6)-linked α-D-glucopyranosyl residues substituted at the O-3 position with α-D-glucopyranosyl branches. In vitro, BP inhibited A549 cell proliferation and increased reactive oxygen species production, thus, promoting the apoptosis of cells. Moreover, BP up-regulated the expression of caspase-3 and the corresponding protein, indicating that it activates the caspase-3 pathway. These findings suggest that the potential of BP as a supplement or drug to treat cancer.

在这项研究中，枯草芽孢杆菌使用牛d寡聚果糖作为碳源生产了一种多糖BP 。通过热水提取，乙醇沉淀，阴离子交换和凝胶过滤色谱法从通过枯草芽孢杆菌发酵的牛d中分离多糖BP 。BP的分子量确定为1.533×10 ⁷ g / mol，由甘露糖，鼠李糖，葡萄糖，半乳糖和阿拉伯糖组成，比例为1：0.12：17.16：0.69：1.10。此外，BP由（1→6）连接的α-D-吡喃葡萄糖基残基的骨架结构构成，该残基在O-3位置被α-D-吡喃葡萄糖基分支取代。体外BP抑制A549细胞增殖并增加活性氧的产生，从而促进细胞凋亡。此外，BP上调了caspase-3和相应蛋白的表达，表明它激活了caspase-3途径。这些发现表明BP作为治疗癌症的补充剂或药物的潜力。