A dual functional polyurethane (PU) film that mimics aspects of blood vessel inner surfaces by combining surface texturing and nitric oxide (NO) release was fabricated through a soft lithography two-stage replication process. The fabrication of submicron textures on the polymer surface was followed by solvent impregnation with the NO donor, S-nitroso-N-acetylpenicillamine (SNAP). An in vitro plasma coagulation assay showed that the biomimetic surface significantly increased the plasma coagulation time and also exhibited reduced platelet adhesion and activation, thereby reducing the risk of blood coagulation and thrombosis. A contact activation assay for coagulation factor XII (FXII) demonstrated that both NO release and surface texturing also reduced FXII contact activation, which contributes to the inhibition of plasma coagulation. The biomimetic surface was also evaluated for bacterial adhesion in plasma and results demonstrate that this combined strategy enables a synergistic effect to reduce bacterial adhesion of Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas aeruginosa microorganisms. The results strongly suggest that the biomimetic modification with surface texturing and NO release provides an effective approach to improve the biocompatibility of polymeric materials in combating thrombosis and microbial infection. STATEMENT OF SIGNIFICANCE: (1) Developed a dual functional polyurethane (PU) film that mimics blood vessel inner surface by combining surface texturing and nitric oxide (NO) release for combatting biomaterial associated thrombosis and microbial infection. (2) Studied the blood coagulation response and bacterial adhesion to such biomimetic PU surfaces, and demonstrated that the combination of surface texturing and NO release synergistically reduced the platelet adhesion and bacterial adhesion in plasma, providing an effective approach to improve the biocompatibility of biomaterials used in blood-contacting medical devices. (3) The NO releasing surface significantly inhibits the plasma coagulation via the reduction of contact activation of FXII, indicating the multifunctional roles of NO in improving the biocompatibility of biomaterials in blood-contacting medical devices.

中文翻译：

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对具有表面纹理和一氧化氮释放作用的仿生聚氨酯生物材料的凝血反应和细菌粘附。

通过软光刻两步复制工艺，通过结合表面纹理和一氧化氮（NO）释放来模仿血管内表面各方面的双功能聚氨酯（PU）膜。在聚合物表面上制备亚微米织构，然后用NO供体S-亚硝基-N-乙酰青霉胺（SNAP）进行溶剂浸渍。体外血浆凝集测定表明，仿生表面显着增加了血浆凝集时间，并且还表现出减少的血小板粘附和活化，从而降低了血液凝集和血栓形成的风险。凝血因子XII（FXII）的接触活化分析表明，NO释放和表面纹理化也降低了FXII接触活化，这有助于抑制血浆凝结。还评估了仿生表面在血浆中的细菌粘附，结果表明该联合策略能够降低表皮葡萄球菌，金黄色葡萄球菌和铜绿假单胞菌微生物的细菌粘附。结果强烈表明，具有表面纹理和NO释放的仿生修饰提供了一种有效的方法来改善聚合物材料在对抗血栓形成和微生物感染方面的生物相容性。重大意义声明：（1）开发了一种双功能聚氨酯（PU）膜，通过结合表面纹理和一氧化氮（NO）释放来模拟血管内表面，以对抗与生物材料相关的血栓形成和微生物感染。（2）研究了仿生PU表面的凝血反应和细菌粘附，证明表面纹理化和NO释放的组合协同降低了血浆中的血小板粘附和细菌粘附，为提高所用生物材料的生物相容性提供了有效途径在接触血液的医疗设备中。（3）NO释放表面通过减少FXII的接触活化而显着抑制血浆凝结，表明NO在改善血液接触医疗器械中生物材料的生物相容性中的多功能作用。提供一种有效的方法来改善血液接触医疗设备中使用的生物材料的生物相容性。（3）NO释放表面通过减少FXII的接触活化而显着抑制血浆凝结，表明NO在改善血液接触医疗器械中生物材料的生物相容性中的多功能作用。提供一种有效的方法来改善血液接触医疗设备中使用的生物材料的生物相容性。（3）NO释放表面通过减少FXII的接触活化而显着抑制血浆凝结，表明NO在改善血液接触医疗器械中生物材料的生物相容性中的多功能作用。