Abstract Soil burial biodegradation behavior of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] was studied based on its copolymer composition determined by thermally assisted hydrolysis and methylation-gas chromatography (THM-GC). The change in copolymer composition of P(3HB-co-3HHx) film samples during soil burial degradation test was monitored in detail by THM-GC. As a result, the copolymer compositions of 3HB units gradually increased, while those of 3HHx units decreased with soil burial time, almost correlating with recovery weight. Based on these results, the biodegradation mechanism of P(3HB-co-3HHx) was elucidated with considering the influence of the crystallinity on the biodegradability. Furthermore, local differences in biodegradability of a given P(3HB-co-3HHx) film after soil burial test were also successfully evaluated from the copolymer compositions obtained by THM-GC.

中文翻译：

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基于热辅助水解和甲基化-气相色谱监测的共聚物组成变化评估聚（3-羟基丁酸酯-co-3-羟基己酸酯）的土壤埋藏生物降解行为

摘要 基于聚(3-羟基丁酸酯-co-3-羟基己酸酯)[P(3HB-co-3HHx)]的共聚组成，通过热辅助水解和甲基化-气相色谱(THM-GC)测定其土壤埋藏生物降解行为. THM-GC详细监测了土壤埋藏降解试验过程中P(3HB-co-3HHx)薄膜样品共聚物组成的变化。结果，3HB单元的共聚物组成逐渐增加，而3HHx单元的共聚物组成随着土壤埋藏时间而减少，几乎与恢复重量相关。基于这些结果，考虑了结晶度对生物降解性的影响，阐明了 P(3HB-co-3HHx) 的生物降解机制。此外，