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Catalase immobilization onto magnetic multi-walled carbon nanotubes: optimization of crucial parameters using response surface methodology
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2018-11-20 00:00:00 , DOI: 10.1039/c8nj03517b Hossain-Ali Rafiee-Pour 1, 2, 3, 4, 5 , Mahboubeh Nejadhosseinian 6, 7, 8, 9 , Masoumeh Firouzi 6, 7, 8, 9 , Saeed Masoum 3, 4, 5, 9, 10
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2018-11-20 00:00:00 , DOI: 10.1039/c8nj03517b Hossain-Ali Rafiee-Pour 1, 2, 3, 4, 5 , Mahboubeh Nejadhosseinian 6, 7, 8, 9 , Masoumeh Firouzi 6, 7, 8, 9 , Saeed Masoum 3, 4, 5, 9, 10
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In this study catalase (CAT) immobilization onto magnetic multi-walled carbon nanotubes (mMWCNTs) was undertaken and response surface methodology (RSM) employed to determine the optimum immobilization conditions. The validity of the predicted values was established by comparing the actual and estimated values of CAT/mMWCNTs activity, 7.39 and 7.48 mM min−1, respectively. The kinetic parameters of the Michaelis–Menten constant (Km; 8.90 mM) and the maximum velocity of the reaction (Vmax; 0.040 s−1) were calculated for CAT/mMWCNTs in comparison with free CAT in buffer solution (7.65 mM and 0.047 s−1). The saturation magnetization (Ms) parameter for mMWCNTs was obtained at around 19.8 emu g−1, which is considerably higher than that for raw MWCNTs (Ms; 0.63 emu g−1) which shows the successful magnetization of MWCNTs. Furthermore, scanning electron microscopy (SEM) showed that enzyme molecules successfully immobilized onto mMWCNTs. The obtained results illustrated that mMWCNTs are potentially able to bind enzyme molecules and reduce the costs of industrial enzyme systems.
中文翻译：
过氧化氢酶固定在磁性多壁碳纳米管上：使用响应表面方法优化关键参数
在这项研究中，将过氧化氢酶（CAT）固定在磁性多壁碳纳米管（mMWCNT）上，并采用响应面方法（RSM）确定最佳固定条件。通过比较CAT / mMWCNTs活性的实际值和估计值分别为7.39和7.48 mM min -1来确定预测值的有效性。与缓冲溶液中的游离CAT（7.65 mM和4.6）相比，计算了CAT / mMWCNTs的Michaelis-Menten常数（K m ; 8.90 mM）和最大反应速度（V max ; 0.040 s -1）的动力学参数。0.047 s -1）。饱和磁化强度（M s）在约19.8鸸鹋克得到了用于mMWCNTs参数-1，这比用于原始多壁碳纳米管显着更高（中号小号; 0.63鸸鹋克-1），其示出了多壁碳纳米管的成功磁化。此外，扫描电子显微镜（SEM）显示酶分子成功地固定在mMWCNTs上。获得的结果说明，mMWCNTs潜在地能够结合酶分子并降低工业酶系统的成本。
更新日期：2018-11-20
中文翻译：
过氧化氢酶固定在磁性多壁碳纳米管上：使用响应表面方法优化关键参数
在这项研究中，将过氧化氢酶（CAT）固定在磁性多壁碳纳米管（mMWCNT）上，并采用响应面方法（RSM）确定最佳固定条件。通过比较CAT / mMWCNTs活性的实际值和估计值分别为7.39和7.48 mM min -1来确定预测值的有效性。与缓冲溶液中的游离CAT（7.65 mM和4.6）相比，计算了CAT / mMWCNTs的Michaelis-Menten常数（K m ; 8.90 mM）和最大反应速度（V max ; 0.040 s -1）的动力学参数。0.047 s -1）。饱和磁化强度（M s）在约19.8鸸鹋克得到了用于mMWCNTs参数-1，这比用于原始多壁碳纳米管显着更高（中号小号; 0.63鸸鹋克-1），其示出了多壁碳纳米管的成功磁化。此外，扫描电子显微镜（SEM）显示酶分子成功地固定在mMWCNTs上。获得的结果说明，mMWCNTs潜在地能够结合酶分子并降低工业酶系统的成本。
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