Glioblastoma (GB) is a highly infiltrative tumor, recurring, in 90% of cases, within a few centimeters of the surgical resection cavity, even with adjuvant chemo/radiotherapy. Residual GB cells left in the margins or infiltrating the brain parenchyma shelter behind the extremely fragile and sensitive brain tissue and may favor recurrence. Tools for eliminating these cells without damaging the brain microenvironment are urgently required. We propose a strategy involving the implantation, into the tumor bed after resection, of a scaffold to concentrate and trap these cells, to facilitate their destruction by targeted therapies, such as stereotactic radiosurgery. We used bacterial cellulose (BC), an easily synthesized and modifiable random nanofibrous biomaterial, to make the trap. We showed that the structure of BC membranes was ideal for trapping tumor cells and that BC implants were biocompatible with brain parenchyma. We also demonstrated the visibility of BC on magnetic resonance imaging, making it possible to follow its fate in clinical situations and to define the target volume for stereotactic radiosurgery more precisely. Furthermore, BC membranes can be loaded with chemoattractants, which were released and attracted tumor cells in vitro. This is of particular interest for trapping GB cells infiltrating tissues within a few centimeters of the resection cavity. Our data suggest that BC membranes could be a scaffold of choice for implantation after surgical resection to trap residual GB cells. STATEMENT OF SIGNIFICANCE: Glioblastoma is a highly infiltrative tumor, recurring, in 90% of cases, within a few centimeters of the surgical resection cavity, even with adjuvant chemo/radiotherapy. Residual tumor cells left in the margins or infiltrating the brain parenchyma shelter behind the extremely fragile and sensitive brain tissue and contribute to the risk of recurrence. Finding tools to eliminate these cells without damaging the brain microenvironment is a real challenge. We propose a strategy involving the implantation, into the walls of the surgical resection cavity, of a scaffold to concentrate and trap the residual tumor cells, to facilitate their destruction by targeted therapies, such as stereotactic radiosurgery.

中文翻译：

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一种新的胶质母细胞瘤细胞捕集器，用于在手术切除后植入。

胶质母细胞瘤（GB）是高度浸润性肿瘤，即使使用辅助化学疗法/放射疗法，也有90％的病例在手术切除腔的几厘米内复发。残留在边缘或浸润在脑实质内的GB细胞残留在极其脆弱和敏感的脑组织后面，并可能有助于复发。迫切需要在不损害大脑微环境的情况下消除这些细胞的工具。我们提出了一种策略，涉及在切除后将支架植入肿瘤床，以浓缩和捕获这些细胞，以促进其通过定向疗法（例如立体定向放射外科手术）的破坏。我们使用细菌纤维素（BC）（一种易于合成且可修改的随机纳米纤维生物材料）制作陷阱。我们表明，BC膜的结构非常适合捕获肿瘤细胞，并且BC植入物与脑实质具有生物相容性。我们还展示了BC在磁共振成像中的可见性，从而有可能在临床情况下追踪其命运，并更精确地定义立体定向放射外科手术的目标体积。此外，BC膜可以装载趋化剂，其在体外被释放并吸引肿瘤细胞。对于将渗入组织的GB细胞捕获在切除腔几厘米之内，这是特别有意义的。我们的数据表明，BC膜可能是手术切除后捕获残留GB细胞的植入支架的选择。重要性声明：胶质母细胞瘤是高度浸润性肿瘤，在90％的病例中复发，即使在辅助化学疗法/放射疗法的情况下，也要在手术切除腔几厘米的范围内。残留在边缘或浸润在脑实质内的残留肿瘤细胞位于极其脆弱和敏感的脑组织后面，并有复发的危险。寻找消除这些细胞而不损害大脑微环境的工具是一个真正的挑战。我们提出了一种策略，该策略涉及将支架植入到手术切除腔壁中，以集中并捕获残留的肿瘤细胞，以利于通过定向疗法（例如立体定向放射外科手术）对其进行破坏。残留在边缘或浸润在脑实质内的残留肿瘤细胞位于极其脆弱和敏感的脑组织后方，并有复发风险。寻找消除这些细胞而不损害大脑微环境的工具是一个真正的挑战。我们提出了一种策略，该策略涉及将支架植入到手术切除腔壁中，以集中并捕获残留的肿瘤细胞，以利于通过定向疗法（例如立体定向放射外科手术）对其进行破坏。残留在边缘或浸润在脑实质内的残留肿瘤细胞位于极其脆弱和敏感的脑组织后方，并有复发风险。寻找消除这些细胞而不损害大脑微环境的工具是一个真正的挑战。我们提出了一种策略，该策略涉及将支架植入到手术切除腔壁中，以集中并捕获残留的肿瘤细胞，以利于通过定向疗法（例如立体定向放射外科手术）对其进行破坏。