Lepidopteran geometrid moth can produce complex Type II sex pheromone components to attract males and trigger mating behavior. Although several sex pheromone components have been identified, it remains unclear whether their physicochemical roles in sex pheromone sensing are the same. Therefore, we utilized tea geometrid (Ectropis obliqua) as an example model to investigate and compare the physicochemical basis of two key Type II sex pheromone components, cis-6,7-epoxy-(3Z,9Z)-3,9-octadecadiene (Z3Z9-6,7-epo-18:Hy) and (Z,Z,Z)-3,6,9-octadecatriene (Z3Z6Z9-18:Hy), interacting with pheromone-binding protein 2 (EoblPBP2) from E. obliqua. Multispectral, thermodynamic, docking, and site-directed mutagenesis indicated that the major sex pheromone component Z3Z9-6,7-epo-18:Hy is more susceptible to pH-tuned than the minor component Z3Z6Z9-18:Hy, whereas Z3Z6Z9-18:Hy seems to be more susceptible to temperature and amino acid mutations than Z3Z9-6,7-epo-18:Hy. Our study suggests that different components of Type II sex pheromone play different binding characters under specific conditions in the physicochemical behavior. This deeply supplements the theoretical knowledge of Type II pheromones involved in the recognition and discrimination in the Lepidopteran sex pheromones family.

中文翻译：

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茶尺metri茶的信息素结合蛋白2与两种II型性信息素成分结合的理化基础和比较

鳞翅目鳞翅目蛾可产生复杂的II型性信息素成分，以吸引雄性并触发交配行为。尽管已鉴定出几种性信息素成分，但尚不清楚它们在性信息素感测中的物理化学作用是否相同。因此，我们以茶几何（Ectropis obliqua）为例，研究和比较了两种关键的II型性信息素成分，即cis-6,7-环氧-（3Z，9Z）-3,9-十八碳二烯（Z 3 Z 9-6,7-epo-18：Hy）和（Z，Z，Z）-3,6,9-十八碳三烯（Z3Z6Z9-18：Hy），与信息素结合蛋白2（Eobl PBP2）相互作用来自E. obliqua。多光谱，热力学，对接和定点诱变表明，主要的信息素组分Z3Z9-6,7-epo-18：Hy比次要组分Z3Z6Z9-18：Hy更容易受到pH调节，而Z3Z6Z9-18 ：Hy似乎比Z3Z9-6,7-epo-18：Hy更容易受到温度和氨基酸突变的影响。我们的研究表明，II型性信息素的不同成分在特定条件下的物理化学行为中具有不同的结合特性。这极大地补充了鳞翅目性信息素家族中涉及识别和区分的II型信息素的理论知识。