We developed a dual-adeno-associated-virus expression system that enables strong and sparse labeling of individual neurons with cell-type and projection specificity. We demonstrated its utility for whole-brain reconstruction of midbrain dopamine neurons and striatum-projecting cortical neurons. We further extended the labeling method for rapid reconstruction in cleared thick brain sections and simultaneous dual-color labeling. This labeling system may facilitate the process of generating mesoscale single-neuron projectomes of mammalian brains.

中文翻译：

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单个神经元的细胞类型特定和投影特定的全脑重建

我们开发了一种双腺相关病毒表达系统，该系统可以对单个神经元进行强而稀疏的标记，并具有细胞类型和投射特异性。我们证明了其可用于中脑多巴胺神经元和纹状体投射皮层神经元的全脑重建。我们进一步扩展了标记方法，以在清除的厚脑组织中快速重建并同时进行双色标记。该标记系统可以促进哺乳动物大脑中尺度单神经元射影的生成过程。